Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20795 | 5' | -52.6 | NC_004689.1 | + | 41774 | 0.67 | 0.843941 |
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Target: 5'- cGCuUGCAGCcgguUGCcgcCCAGuGCCUUgACGCc -3' miRNA: 3'- -CG-ACGUUG----ACGu--GGUC-UGGAAgUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 44887 | 0.67 | 0.860938 |
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Target: 5'- cGCUGCGgaGCuUGCGCggauCGGGCCUgccggUCACcGCu -3' miRNA: 3'- -CGACGU--UG-ACGUG----GUCUGGA-----AGUG-CG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 48918 | 0.71 | 0.616209 |
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Target: 5'- aCUGCGACUGCGCgagCAGGgCUUCG-GCc -3' miRNA: 3'- cGACGUUGACGUG---GUCUgGAAGUgCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 51341 | 0.67 | 0.86011 |
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Target: 5'- cCUGCGaaACUGCaaaccccGCCAgGGCUUUC-CGCa -3' miRNA: 3'- cGACGU--UGACG-------UGGU-CUGGAAGuGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 51815 | 0.74 | 0.466461 |
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Target: 5'- cCUGCAACcaCGCCAGACCcUCGuCGCu -3' miRNA: 3'- cGACGUUGacGUGGUCUGGaAGU-GCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 55098 | 0.68 | 0.777867 |
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Target: 5'- uUUGCAcucgcguucgaACUGCGCguGAUCaUUCACGUu -3' miRNA: 3'- cGACGU-----------UGACGUGguCUGG-AAGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 60938 | 0.68 | 0.797674 |
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Target: 5'- cGCUGguGCUGC-CCAGGugg-CGCGUg -3' miRNA: 3'- -CGACguUGACGuGGUCUggaaGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 62973 | 0.66 | 0.899079 |
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Target: 5'- --aGCcGC-GCACCGGuACCgggaaaUCACGCg -3' miRNA: 3'- cgaCGuUGaCGUGGUC-UGGa-----AGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 69991 | 0.66 | 0.876967 |
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Target: 5'- cGCUcGCGAUguaCACCAuucGACUUgaUCACGCg -3' miRNA: 3'- -CGA-CGUUGac-GUGGU---CUGGA--AGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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