Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2085 | 5' | -52.4 | NC_001348.1 | + | 64959 | 0.7 | 0.864676 |
Target: 5'- uUAAUGGAgacguuucgccugaaGACGCACaACGCCUCaCGgCu -3' miRNA: 3'- -GUUACCU---------------UUGUGUGcUGCGGAG-GCgG- -5' |
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2085 | 5' | -52.4 | NC_001348.1 | + | 4830 | 0.7 | 0.845405 |
Target: 5'- gAAUGGcccAAACAUACGACGUaUUCGUCg -3' miRNA: 3'- gUUACC---UUUGUGUGCUGCGgAGGCGG- -5' |
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2085 | 5' | -52.4 | NC_001348.1 | + | 23112 | 0.71 | 0.810705 |
Target: 5'- --uUGGAAACACACaGugGaUUCCGUCu -3' miRNA: 3'- guuACCUUUGUGUG-CugCgGAGGCGG- -5' |
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2085 | 5' | -52.4 | NC_001348.1 | + | 60621 | 0.72 | 0.76353 |
Target: 5'- uGAUGGAGAaACuCGACGUCUCCGa- -3' miRNA: 3'- gUUACCUUUgUGuGCUGCGGAGGCgg -5' |
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2085 | 5' | -52.4 | NC_001348.1 | + | 87010 | 0.77 | 0.519262 |
Target: 5'- --cUGGGAACGCuCGugGCCUgugcuacguugggaaCCGCCg -3' miRNA: 3'- guuACCUUUGUGuGCugCGGA---------------GGCGG- -5' |
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2085 | 5' | -52.4 | NC_001348.1 | + | 47708 | 1.13 | 0.003096 |
Target: 5'- gCAAUGGAAACACACGACGCCUCCGCCg -3' miRNA: 3'- -GUUACCUUUGUGUGCUGCGGAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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