Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2085 | 5' | -52.4 | NC_001348.1 | + | 108710 | 0.66 | 0.975714 |
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Target: 5'- aCGAUGGGgaaacgGGCAgaggUACGACcCCUCCGUUc -3' miRNA: 3'- -GUUACCU------UUGU----GUGCUGcGGAGGCGG- -5' |
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| 2085 | 5' | -52.4 | NC_001348.1 | + | 29497 | 0.66 | 0.975714 |
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Target: 5'- ---aGGAcGCAUACGA-GCUgaaaaugCCGCCu -3' miRNA: 3'- guuaCCUuUGUGUGCUgCGGa------GGCGG- -5' |
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| 2085 | 5' | -52.4 | NC_001348.1 | + | 60646 | 0.66 | 0.975714 |
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Target: 5'- aAAUGG-GACACAa-AUGCCUuuGUCg -3' miRNA: 3'- gUUACCuUUGUGUgcUGCGGAggCGG- -5' |
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| 2085 | 5' | -52.4 | NC_001348.1 | + | 124213 | 0.66 | 0.978206 |
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Target: 5'- gGGUGGAAGCACGaGugG-UUCUGCg -3' miRNA: 3'- gUUACCUUUGUGUgCugCgGAGGCGg -5' |
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| 2085 | 5' | -52.4 | NC_001348.1 | + | 93365 | 0.66 | 0.978206 |
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Target: 5'- --cUGGAAgGCAUugGGC-CCUCCGg- -3' miRNA: 3'- guuACCUU-UGUGugCUGcGGAGGCgg -5' |
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| 2085 | 5' | -52.4 | NC_001348.1 | + | 87010 | 0.77 | 0.519262 |
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Target: 5'- --cUGGGAACGCuCGugGCCUgugcuacguugggaaCCGCCg -3' miRNA: 3'- guuACCUUUGUGuGCugCGGA---------------GGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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