Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21042 | 3' | -57 | NC_004745.1 | + | 19535 | 0.69 | 0.295372 |
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Target: 5'- uUCGCCAgCGCCAUauucagcgugacCGGcGuuGCCGCCu -3' miRNA: 3'- -GGUGGUaGUGGUA------------GUCuCggCGGCGG- -5' |
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| 21042 | 3' | -57 | NC_004745.1 | + | 9598 | 0.7 | 0.287888 |
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Target: 5'- gCCACUGaaGCCAgUCAGuGCacauGCCGCCg -3' miRNA: 3'- -GGUGGUagUGGU-AGUCuCGg---CGGCGG- -5' |
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| 21042 | 3' | -57 | NC_004745.1 | + | 4613 | 0.7 | 0.273368 |
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Target: 5'- aCCGCCAcauccugCAgCAUCGGAuuGCUGCUGCg -3' miRNA: 3'- -GGUGGUa------GUgGUAGUCU--CGGCGGCGg -5' |
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| 21042 | 3' | -57 | NC_004745.1 | + | 9880 | 0.7 | 0.250703 |
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Target: 5'- aCCACCggCACCGuggcaguaacagaaUCAGaAGCCGUcaCGCUg -3' miRNA: 3'- -GGUGGuaGUGGU--------------AGUC-UCGGCG--GCGG- -5' |
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| 21042 | 3' | -57 | NC_004745.1 | + | 7825 | 1.12 | 0.000192 |
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Target: 5'- gCCACCAUCACCAUCAGAGCCGCCGCCc -3' miRNA: 3'- -GGUGGUAGUGGUAGUCUCGGCGGCGG- -5' |
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| 21042 | 3' | -57 | NC_004745.1 | + | 28807 | 0.66 | 0.486393 |
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Target: 5'- aCACCGUCAUCAacCAGAuuaagcacuGCUGCgGCg -3' miRNA: 3'- gGUGGUAGUGGUa-GUCU---------CGGCGgCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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