Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21042 | 3' | -57 | NC_004745.1 | + | 19953 | 0.7 | 0.280553 |
Target: 5'- -aACCAUUucggggaggagGCCAgcucAGCCGCCGCCg -3' miRNA: 3'- ggUGGUAG-----------UGGUagucUCGGCGGCGG- -5' |
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21042 | 3' | -57 | NC_004745.1 | + | 27726 | 0.75 | 0.116177 |
Target: 5'- aCACCAUUGCCGguauuGAGCguuaCGCCGCCg -3' miRNA: 3'- gGUGGUAGUGGUagu--CUCG----GCGGCGG- -5' |
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21042 | 3' | -57 | NC_004745.1 | + | 28471 | 0.7 | 0.280553 |
Target: 5'- -uGCCAg-ACUGUCAGAGUCGCCcguaGCCc -3' miRNA: 3'- ggUGGUagUGGUAGUCUCGGCGG----CGG- -5' |
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21042 | 3' | -57 | NC_004745.1 | + | 28657 | 0.66 | 0.486393 |
Target: 5'- aUCGCCG-CGCC-UCGGAGUC-CUGUCa -3' miRNA: 3'- -GGUGGUaGUGGuAGUCUCGGcGGCGG- -5' |
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21042 | 3' | -57 | NC_004745.1 | + | 28719 | 0.67 | 0.396911 |
Target: 5'- aCCGgcucCCA-CACCuUCuGAGCaCGCCGCa -3' miRNA: 3'- -GGU----GGUaGUGGuAGuCUCG-GCGGCGg -5' |
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21042 | 3' | -57 | NC_004745.1 | + | 28807 | 0.66 | 0.486393 |
Target: 5'- aCACCGUCAUCAacCAGAuuaagcacuGCUGCgGCg -3' miRNA: 3'- gGUGGUAGUGGUa-GUCU---------CGGCGgCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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