Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21052 | 5' | -55.3 | NC_004745.1 | + | 4067 | 0.66 | 0.550339 |
Target: 5'- cGG-ACUGAcUGAcGCCGGUCAGCAg-- -3' miRNA: 3'- -CCgUGGCU-ACUaCGGUCAGUCGUggu -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 5170 | 0.66 | 0.550339 |
Target: 5'- aGCGCCacAUGAUGCgGGUCucGGCAgCGa -3' miRNA: 3'- cCGUGGc-UACUACGgUCAG--UCGUgGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 177 | 0.66 | 0.539086 |
Target: 5'- aGGCGgCGAUGuucaGCCGuuGUCAGUGuCCAg -3' miRNA: 3'- -CCGUgGCUACua--CGGU--CAGUCGU-GGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 1739 | 0.66 | 0.539086 |
Target: 5'- uGGCACCGccgGUGGUgGCCGGU-GGCAaauUCAg -3' miRNA: 3'- -CCGUGGC---UACUA-CGGUCAgUCGU---GGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 6924 | 0.66 | 0.516826 |
Target: 5'- gGGUaucGCCGGuuaucaggugauUGAgauUGCCAuUCAGCGCCGc -3' miRNA: 3'- -CCG---UGGCU------------ACU---ACGGUcAGUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 1404 | 0.66 | 0.505834 |
Target: 5'- --aGCUGGUGAUGUCGGU-GGCGCUg -3' miRNA: 3'- ccgUGGCUACUACGGUCAgUCGUGGu -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 20855 | 0.66 | 0.505834 |
Target: 5'- uGGCACCGGUGGUuaUAuucCGGCgACCAg -3' miRNA: 3'- -CCGUGGCUACUAcgGUca-GUCG-UGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 1106 | 0.67 | 0.483089 |
Target: 5'- cGGCA--GAUGGUaugagccGCCGGUCAGCuuuACCGg -3' miRNA: 3'- -CCGUggCUACUA-------CGGUCAGUCG---UGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 9839 | 0.67 | 0.473492 |
Target: 5'- cGGUGCCGGUGGUuaUGGUgaaAGCAUCAa -3' miRNA: 3'- -CCGUGGCUACUAcgGUCAg--UCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 20453 | 0.67 | 0.473492 |
Target: 5'- cGCACCGAUGGccGUCAuaaCGGCACUAc -3' miRNA: 3'- cCGUGGCUACUa-CGGUca-GUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 18396 | 0.67 | 0.452518 |
Target: 5'- cGGCACCGAUGcUGaCCgagcaggaGGUCGccGCGCUg -3' miRNA: 3'- -CCGUGGCUACuAC-GG--------UCAGU--CGUGGu -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 4281 | 0.67 | 0.442222 |
Target: 5'- uGGCAUCGGUGucaCCGGcuccaucgcCAGCACCAc -3' miRNA: 3'- -CCGUGGCUACuacGGUCa--------GUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 6690 | 0.67 | 0.442222 |
Target: 5'- gGGCAguUCGAcggcGGUGCCGuGUggCAGCACCAc -3' miRNA: 3'- -CCGU--GGCUa---CUACGGU-CA--GUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 21805 | 0.68 | 0.422036 |
Target: 5'- cGGCAgcCCGAcc-UGCCcGUCGGCAUCGu -3' miRNA: 3'- -CCGU--GGCUacuACGGuCAGUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 9613 | 0.68 | 0.379649 |
Target: 5'- cGGCA-CGAUGGgucagccacugaaGCCAGUCAGUGCaCAu -3' miRNA: 3'- -CCGUgGCUACUa------------CGGUCAGUCGUG-GU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 8298 | 0.69 | 0.356869 |
Target: 5'- gGGCGCUGGUGcaaccgGCgcacagcaucaggcaGGUCAGCACCGu -3' miRNA: 3'- -CCGUGGCUACua----CGg--------------UCAGUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 8474 | 0.69 | 0.355978 |
Target: 5'- gGGCGCUGAcUGAUGCC-GUCacGGUGCUg -3' miRNA: 3'- -CCGUGGCU-ACUACGGuCAG--UCGUGGu -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 18438 | 0.69 | 0.355978 |
Target: 5'- aGCAUCGGUGccgucAUGCgCGG-CAGCACCu -3' miRNA: 3'- cCGUGGCUAC-----UACG-GUCaGUCGUGGu -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 2910 | 0.69 | 0.338488 |
Target: 5'- cGGCACgGAaaaccccUGCCAGUacagCAGCGCCGc -3' miRNA: 3'- -CCGUGgCUacu----ACGGUCA----GUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 11977 | 0.71 | 0.282266 |
Target: 5'- cGGUGCCG--GAUGCCGGUCAGacgaaACUg -3' miRNA: 3'- -CCGUGGCuaCUACGGUCAGUCg----UGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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