miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21158 3' -52.7 NC_004775.1 + 14034 0.66 0.779035
Target:  5'- uGGCGCaaugGCagaggCUACCugUCgCAUCGCCg -3'
miRNA:   3'- gUCGCG----CGaaaa-GAUGG--AG-GUAGCGG- -5'
21158 3' -52.7 NC_004775.1 + 4882 0.66 0.725044
Target:  5'- uGGCucccuGUGCUgcggcCUGCC-CCAUUGCCa -3'
miRNA:   3'- gUCG-----CGCGAaaa--GAUGGaGGUAGCGG- -5'
21158 3' -52.7 NC_004775.1 + 1275 0.68 0.657056
Target:  5'- uCAGCGCGCcuucugCUACUUCCcacaccagAUCGgCa -3'
miRNA:   3'- -GUCGCGCGaaaa--GAUGGAGG--------UAGCgG- -5'
21158 3' -52.7 NC_004775.1 + 21782 0.7 0.531681
Target:  5'- uGGCGCuGCUUUUUcACCgCCGUCuGCUa -3'
miRNA:   3'- gUCGCG-CGAAAAGaUGGaGGUAG-CGG- -5'
21158 3' -52.7 NC_004775.1 + 32960 0.7 0.520611
Target:  5'- gCGGCGgccUGCUUcUCUACagUCCAUuCGCCa -3'
miRNA:   3'- -GUCGC---GCGAAaAGAUGg-AGGUA-GCGG- -5'
21158 3' -52.7 NC_004775.1 + 18473 0.7 0.487974
Target:  5'- uGGCaGCGCUUggagaUCUACCgCCugagCGCCu -3'
miRNA:   3'- gUCG-CGCGAAa----AGAUGGaGGua--GCGG- -5'
21158 3' -52.7 NC_004775.1 + 29532 1.13 0.000569
Target:  5'- aCAGCGCGCUUUUCUACCUCCAUCGCCg -3'
miRNA:   3'- -GUCGCGCGAAAAGAUGGAGGUAGCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.