Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21176 | 5' | -42.7 | NC_004777.1 | + | 19503 | 0.66 | 0.999504 |
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Target: 5'- aGUGAGUCGUAUUAa-----AUGC-CCg -3' miRNA: 3'- -CACUCAGCAUAAUaaagaaUGCGaGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 5928 | 0.66 | 0.999362 |
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Target: 5'- aGUGAGUCGUAUUAa-----GCGUgaccaUCCg -3' miRNA: 3'- -CACUCAGCAUAAUaaagaaUGCG-----AGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 16247 | 0.68 | 0.997555 |
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Target: 5'- aGUGAGUCGUAUUAa--UUUAgGgaCCu -3' miRNA: 3'- -CACUCAGCAUAAUaaaGAAUgCgaGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 30154 | 0.68 | 0.996316 |
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Target: 5'- -aGAGUCGccacaaaggggCUUACGUUCCu -3' miRNA: 3'- caCUCAGCauaauaaa---GAAUGCGAGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 5822 | 0.71 | 0.979454 |
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Target: 5'- ----aUCGUAggAUgcCUUGCGCUCCa -3' miRNA: 3'- cacucAGCAUaaUAaaGAAUGCGAGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 24899 | 0.71 | 0.970178 |
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Target: 5'- aGUGAGUCGUAUUaAUUUCUa--GCcaCCa -3' miRNA: 3'- -CACUCAGCAUAA-UAAAGAaugCGa-GG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 7193 | 0.71 | 0.970178 |
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Target: 5'- aGUGAGUCGUAUUA---CUUugGUgCUa -3' miRNA: 3'- -CACUCAGCAUAAUaaaGAAugCGaGG- -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 4105 | 0.74 | 0.911704 |
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Target: 5'- aGUGAGUCGUAUUAaUUUgacgUUACGCa-- -3' miRNA: 3'- -CACUCAGCAUAAUaAAG----AAUGCGagg -5' |
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| 21176 | 5' | -42.7 | NC_004777.1 | + | 32184 | 1.14 | 0.007468 |
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Target: 5'- aGUGAGUCGUAUUAUUUCUUACGCUCCc -3' miRNA: 3'- -CACUCAGCAUAAUAAAGAAUGCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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