Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21184 | 3' | -56.3 | NC_004778.3 | + | 112814 | 0.66 | 0.892509 |
Target: 5'- gUUGUGCuCGuUUGGCACCGgaaGCUcGGg -3' miRNA: 3'- -AGCACG-GC-AGCCGUGGCaugCGAaCU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 5137 | 0.66 | 0.892509 |
Target: 5'- cCGUGCCcacGcCGGCGCC--GCGCUUc- -3' miRNA: 3'- aGCACGG---CaGCCGUGGcaUGCGAAcu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 77915 | 0.66 | 0.885715 |
Target: 5'- gCGUGCacaucCGUCGGCAgCCuaACGCUgauUGAu -3' miRNA: 3'- aGCACG-----GCAGCCGU-GGcaUGCGA---ACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 9895 | 0.66 | 0.878695 |
Target: 5'- -aGUGCCGcCcgGGCGCggUGUGCGCUuuagUGAa -3' miRNA: 3'- agCACGGCaG--CCGUG--GCAUGCGA----ACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 32276 | 0.66 | 0.878695 |
Target: 5'- aCGcugGCCGgCGGCGCCGUGucgggcuggUGCUUa- -3' miRNA: 3'- aGCa--CGGCaGCCGUGGCAU---------GCGAAcu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 40874 | 0.66 | 0.878695 |
Target: 5'- aUGUGCUuuUCGGCguccacauuGCCGUacaaaaacauGCGCUUGAa -3' miRNA: 3'- aGCACGGc-AGCCG---------UGGCA----------UGCGAACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 45319 | 0.66 | 0.871455 |
Target: 5'- ---cGCCGUCcGCugUGUGCGCUc-- -3' miRNA: 3'- agcaCGGCAGcCGugGCAUGCGAacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 32547 | 0.66 | 0.864 |
Target: 5'- cCGcGaCGaCGGCGCCGUACGCg--- -3' miRNA: 3'- aGCaCgGCaGCCGUGGCAUGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 51565 | 0.66 | 0.856335 |
Target: 5'- cCGUGUCGUUG--ACCGUACGgaUGGu -3' miRNA: 3'- aGCACGGCAGCcgUGGCAUGCgaACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 59221 | 0.67 | 0.848466 |
Target: 5'- cCGUGCUGUcCGGCGCUuggGCGCc--- -3' miRNA: 3'- aGCACGGCA-GCCGUGGca-UGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 64671 | 0.67 | 0.848466 |
Target: 5'- aUUG-GCCaUCGGCGCCGUGUGCa--- -3' miRNA: 3'- -AGCaCGGcAGCCGUGGCAUGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 64770 | 0.67 | 0.840401 |
Target: 5'- cCGUGCacaCGGCGCCGauggccaaUAUGCUUGu -3' miRNA: 3'- aGCACGgcaGCCGUGGC--------AUGCGAACu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 107450 | 0.67 | 0.832146 |
Target: 5'- -gGUGCCGUCGGauuUACUGaGCGCg--- -3' miRNA: 3'- agCACGGCAGCC---GUGGCaUGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 61361 | 0.67 | 0.823708 |
Target: 5'- aUGUGCgGaCGGCGCCG-GCGCg--- -3' miRNA: 3'- aGCACGgCaGCCGUGGCaUGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 50869 | 0.67 | 0.823708 |
Target: 5'- --aUGCUGUUGGC--CGUGCGCUUGc -3' miRNA: 3'- agcACGGCAGCCGugGCAUGCGAACu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 49021 | 0.67 | 0.815095 |
Target: 5'- -gGUGCgGcUGGCGCCGUAC-UUUGAc -3' miRNA: 3'- agCACGgCaGCCGUGGCAUGcGAACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 127069 | 0.67 | 0.815095 |
Target: 5'- gCGUGCCGUCGuGCAcgcCCGgcgagugcucGCGUUUGc -3' miRNA: 3'- aGCACGGCAGC-CGU---GGCa---------UGCGAACu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 62693 | 0.67 | 0.815095 |
Target: 5'- -aGUGagGUCGGCGCCG-ACGCa--- -3' miRNA: 3'- agCACggCAGCCGUGGCaUGCGaacu -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 36466 | 0.67 | 0.815095 |
Target: 5'- -gGUGUgGUgGGCGCCaccacGCGCUUGGc -3' miRNA: 3'- agCACGgCAgCCGUGGca---UGCGAACU- -5' |
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21184 | 3' | -56.3 | NC_004778.3 | + | 86807 | 0.67 | 0.815095 |
Target: 5'- cUCGUGCacCGUCGGCGgCGgaACGCa--- -3' miRNA: 3'- -AGCACG--GCAGCCGUgGCa-UGCGaacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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