miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21187 3' -49.6 NC_004778.3 + 6393 0.77 0.631936
Target:  5'- cGUCGuaaaacGCGUUGCUGGCCCGcCg -3'
miRNA:   3'- uCAGCuauaa-CGUAAUGACCGGGCuG- -5'
21187 3' -49.6 NC_004778.3 + 45411 0.66 0.994779
Target:  5'- uGUCGcUGaUGCuguuaguuUUGCUGGCgCGGCg -3'
miRNA:   3'- uCAGCuAUaACGu-------AAUGACCGgGCUG- -5'
21187 3' -49.6 NC_004778.3 + 48393 0.7 0.935289
Target:  5'- cAGuUUGGUGUaGUucUUGCUGGCCCGAa -3'
miRNA:   3'- -UC-AGCUAUAaCGu-AAUGACCGGGCUg -5'
21187 3' -49.6 NC_004778.3 + 48964 0.69 0.954151
Target:  5'- gGGUCGAUGcgcUGCcagcgcacguacAUcacgaACUGGCCCGACc -3'
miRNA:   3'- -UCAGCUAUa--ACG------------UAa----UGACCGGGCUG- -5'
21187 3' -49.6 NC_004778.3 + 49562 0.71 0.918316
Target:  5'- cGUCGcggaagcacuuGUAUUGCcuagcgACUGGCaCCGACa -3'
miRNA:   3'- uCAGC-----------UAUAACGuaa---UGACCG-GGCUG- -5'
21187 3' -49.6 NC_004778.3 + 115191 0.67 0.984168
Target:  5'- cGGUCGAacaggaUGUUGCGaucgcuUUGGCCgGGCa -3'
miRNA:   3'- -UCAGCU------AUAACGUaau---GACCGGgCUG- -5'
21187 3' -49.6 NC_004778.3 + 121843 1.11 0.00646
Target:  5'- aAGUCGAUAUUGCAUUACUGGCCCGACg -3'
miRNA:   3'- -UCAGCUAUAACGUAAUGACCGGGCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.