Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21187 | 3' | -49.6 | NC_004778.3 | + | 6393 | 0.77 | 0.631936 |
Target: 5'- cGUCGuaaaacGCGUUGCUGGCCCGcCg -3' miRNA: 3'- uCAGCuauaa-CGUAAUGACCGGGCuG- -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 49562 | 0.71 | 0.918316 |
Target: 5'- cGUCGcggaagcacuuGUAUUGCcuagcgACUGGCaCCGACa -3' miRNA: 3'- uCAGC-----------UAUAACGuaa---UGACCG-GGCUG- -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 48964 | 0.69 | 0.954151 |
Target: 5'- gGGUCGAUGcgcUGCcagcgcacguacAUcacgaACUGGCCCGACc -3' miRNA: 3'- -UCAGCUAUa--ACG------------UAa----UGACCGGGCUG- -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 45411 | 0.66 | 0.994779 |
Target: 5'- uGUCGcUGaUGCuguuaguuUUGCUGGCgCGGCg -3' miRNA: 3'- uCAGCuAUaACGu-------AAUGACCGgGCUG- -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 115191 | 0.67 | 0.984168 |
Target: 5'- cGGUCGAacaggaUGUUGCGaucgcuUUGGCCgGGCa -3' miRNA: 3'- -UCAGCU------AUAACGUaau---GACCGGgCUG- -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 48393 | 0.7 | 0.935289 |
Target: 5'- cAGuUUGGUGUaGUucUUGCUGGCCCGAa -3' miRNA: 3'- -UC-AGCUAUAaCGu-AAUGACCGGGCUg -5' |
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21187 | 3' | -49.6 | NC_004778.3 | + | 121843 | 1.11 | 0.00646 |
Target: 5'- aAGUCGAUAUUGCAUUACUGGCCCGACg -3' miRNA: 3'- -UCAGCUAUAACGUAAUGACCGGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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