Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21191 | 3' | -48.2 | NC_004778.3 | + | 92785 | 0.66 | 0.999098 |
Target: 5'- cGACUGCguGGaCGAGguGGUgcUGGGCa -3' miRNA: 3'- aCUGGUGguCCaGCUUguUCA--AUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 111293 | 0.66 | 0.999098 |
Target: 5'- uUGGCCGCCGuGGU-GAGCGuuUUGauGGCa -3' miRNA: 3'- -ACUGGUGGU-CCAgCUUGUucAAU--UCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 56743 | 0.66 | 0.999098 |
Target: 5'- aUGACCAUCAaaUCGAACAAcacgugaugcuuGUugUAGGCa -3' miRNA: 3'- -ACUGGUGGUccAGCUUGUU------------CA--AUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 56421 | 0.66 | 0.998893 |
Target: 5'- aGGCUggGCgAGGacucgUUGAGCGAGUUAAGg -3' miRNA: 3'- aCUGG--UGgUCC-----AGCUUGUUCAAUUCg -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 102253 | 0.66 | 0.998893 |
Target: 5'- cUGaACCGCCGGG---GACAGGUUGuuguaguuGGCg -3' miRNA: 3'- -AC-UGGUGGUCCagcUUGUUCAAU--------UCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 72278 | 0.66 | 0.998649 |
Target: 5'- ---aCGCuCAGGUCGAACAGcacguuuuuGUcGAGCa -3' miRNA: 3'- acugGUG-GUCCAGCUUGUU---------CAaUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 79036 | 0.67 | 0.998022 |
Target: 5'- cGaACCGCCA--UUGAACAAGUgcuGCg -3' miRNA: 3'- aC-UGGUGGUccAGCUUGUUCAauuCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 93688 | 0.67 | 0.997627 |
Target: 5'- uUGuCCGCCAGcUCGGGCGauuGGUacaAAGCg -3' miRNA: 3'- -ACuGGUGGUCcAGCUUGU---UCAa--UUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 95856 | 0.67 | 0.997627 |
Target: 5'- cGGCCuCCAcGGUCcagu-GGUUAAGCa -3' miRNA: 3'- aCUGGuGGU-CCAGcuuguUCAAUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 29827 | 0.67 | 0.997627 |
Target: 5'- uUGACagguCguGGUCGAGCAccacaaauucguAGUcGAGCa -3' miRNA: 3'- -ACUGgu--GguCCAGCUUGU------------UCAaUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 110997 | 0.67 | 0.997627 |
Target: 5'- cGGCCACguaGUCGAGCAAGUg---- -3' miRNA: 3'- aCUGGUGgucCAGCUUGUUCAauucg -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 94473 | 0.67 | 0.997119 |
Target: 5'- gUGGCCGCCA--UCGAAaucCGAGUUGcaacguuGGCa -3' miRNA: 3'- -ACUGGUGGUccAGCUU---GUUCAAU-------UCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 64985 | 0.67 | 0.996639 |
Target: 5'- cGGCC-UCGGcGUCGAACGuguAGUUGucggGGCa -3' miRNA: 3'- aCUGGuGGUC-CAGCUUGU---UCAAU----UCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 43440 | 0.67 | 0.996639 |
Target: 5'- cGACCACgAGcGUUGcGACAAGcgcguugAAGCg -3' miRNA: 3'- aCUGGUGgUC-CAGC-UUGUUCaa-----UUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 72495 | 0.67 | 0.996639 |
Target: 5'- -uGCCGCC--GUCGGugGcGUUGAGCg -3' miRNA: 3'- acUGGUGGucCAGCUugUuCAAUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 95811 | 0.68 | 0.995258 |
Target: 5'- -uGCCGCCgugaaacuggcgcAGGUCGAgugGCAGGUUGuguuuGCc -3' miRNA: 3'- acUGGUGG-------------UCCAGCU---UGUUCAAUu----CG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 560 | 0.68 | 0.99454 |
Target: 5'- cGGCUACCGGGgCGGugucuGCGGGUcUGGGUu -3' miRNA: 3'- aCUGGUGGUCCaGCU-----UGUUCA-AUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 40152 | 0.68 | 0.993641 |
Target: 5'- cGGCUACCAuGUCGuagucCAcGUUGGGCa -3' miRNA: 3'- aCUGGUGGUcCAGCuu---GUuCAAUUCG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 6085 | 0.68 | 0.992627 |
Target: 5'- -cACCGCCAGGUUGA---GGUUuuuGCu -3' miRNA: 3'- acUGGUGGUCCAGCUuguUCAAuu-CG- -5' |
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21191 | 3' | -48.2 | NC_004778.3 | + | 120495 | 0.68 | 0.991489 |
Target: 5'- ---aCACCGGGU---GCAGGUUGAGUa -3' miRNA: 3'- acugGUGGUCCAgcuUGUUCAAUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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