Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21215 | 5' | -57.2 | NC_004778.3 | + | 101017 | 0.66 | 0.782019 |
Target: 5'- -aGCGCuGCAGCgcuucaaaacUGCCGGgaaUGGUuuuGAGGg -3' miRNA: 3'- aaCGCGuCGUCG----------GCGGCC---AUCA---UUCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 2454 | 0.66 | 0.772559 |
Target: 5'- -aGUGCGGCAcGCCGCUGGUu------ -3' miRNA: 3'- aaCGCGUCGU-CGGCGGCCAucauucc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 88337 | 0.66 | 0.772559 |
Target: 5'- -aGCGCAGCugcguGCUGCacaaaugcgucgUGGUGGUGuuGGGc -3' miRNA: 3'- aaCGCGUCGu----CGGCG------------GCCAUCAU--UCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 78390 | 0.67 | 0.752282 |
Target: 5'- -aGCGCAGUugccacaacaacuGGCCGCCucuGUGGUAGa- -3' miRNA: 3'- aaCGCGUCG-------------UCGGCGGc--CAUCAUUcc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 102233 | 0.68 | 0.717461 |
Target: 5'- -aGCGCGGCAGCgaacuuuugcugaacCGCCGG-GGacAGGu -3' miRNA: 3'- aaCGCGUCGUCG---------------GCGGCCaUCauUCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 34239 | 0.68 | 0.672424 |
Target: 5'- -cGgGCAGCAGcCCGUCGGcAGccGGGc -3' miRNA: 3'- aaCgCGUCGUC-GGCGGCCaUCauUCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 30816 | 0.68 | 0.662069 |
Target: 5'- -gGgGCAGCucgaccGGCCGCUggaGGUGGUAGGc -3' miRNA: 3'- aaCgCGUCG------UCGGCGG---CCAUCAUUCc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 95280 | 0.69 | 0.630887 |
Target: 5'- cUGCGCAGCuugucGCCGCCGGc------- -3' miRNA: 3'- aACGCGUCGu----CGGCGGCCaucauucc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 111278 | 0.69 | 0.620484 |
Target: 5'- aUGCGCguuAGCgccuuGGCCGCC-GUGGUGAGc -3' miRNA: 3'- aACGCG---UCG-----UCGGCGGcCAUCAUUCc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 96768 | 0.7 | 0.568759 |
Target: 5'- -cGCGCAGCuGCCGCggCGcGUGGUGcguaaugugcAGGu -3' miRNA: 3'- aaCGCGUCGuCGGCG--GC-CAUCAU----------UCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 25654 | 0.71 | 0.528161 |
Target: 5'- cUUGCGCGGgGGCCGgCGGcguGUGGGu -3' miRNA: 3'- -AACGCGUCgUCGGCgGCCau-CAUUCc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 95779 | 0.71 | 0.50826 |
Target: 5'- -cGCGCGGCAGCgCGCCGGc------- -3' miRNA: 3'- aaCGCGUCGUCG-GCGGCCaucauucc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 32839 | 0.72 | 0.469469 |
Target: 5'- -cGCGCuAGCGGCCGCCGcGcUGGcUAgcAGGg -3' miRNA: 3'- aaCGCG-UCGUCGGCGGC-C-AUC-AU--UCC- -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 112214 | 0.72 | 0.460007 |
Target: 5'- -cGCGCauAGCGGCCGCgCGGUccaGGUGAu- -3' miRNA: 3'- aaCGCG--UCGUCGGCG-GCCA---UCAUUcc -5' |
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21215 | 5' | -57.2 | NC_004778.3 | + | 86337 | 1.07 | 0.00215 |
Target: 5'- cUUGCGCAGCAGCCGCCGGUAGUAAGGc -3' miRNA: 3'- -AACGCGUCGUCGGCGGCCAUCAUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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