Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21236 | 3' | -47.5 | NC_004778.3 | + | 66747 | 0.95 | 0.114119 |
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Target: 5'- gUCUAAAGAUAGCACUCGCUUUUCGa- -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCcc -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 20004 | 1.02 | 0.047041 |
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Target: 5'- gUUUAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 67007 | 1.05 | 0.030369 |
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Target: 5'- cCUAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- aGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 20160 | 1.12 | 0.010828 |
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Target: 5'- gUCCAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 66813 | 1.12 | 0.010828 |
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Target: 5'- gUCCAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 66879 | 1.12 | 0.010828 |
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Target: 5'- gUCCAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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