Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
215 | 3' | -49.5 | AC_000008.1 | + | 8124 | 0.66 | 0.922544 |
Target: 5'- -aUUGAUCCGGAuagugGC-CGGGAggUcCCg -3' miRNA: 3'- gaAACUGGGCCU-----UGcGCCUUuuGuGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 23443 | 0.66 | 0.91609 |
Target: 5'- -gUUGGCCugcggcugcugcCGGAACGCgucaGGAuuGCGCg -3' miRNA: 3'- gaAACUGG------------GCCUUGCG----CCUuuUGUGg -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 16970 | 0.66 | 0.909326 |
Target: 5'- ---cGuCUCGGuGGCGCGGGcaaacuGCACCa -3' miRNA: 3'- gaaaCuGGGCC-UUGCGCCUuu----UGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 10464 | 0.66 | 0.902255 |
Target: 5'- ---cGGCCaCGGAGCGCucguuGAAgGCCg -3' miRNA: 3'- gaaaCUGG-GCCUUGCGccu--UUUgUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 12119 | 0.66 | 0.902255 |
Target: 5'- aCUUUGACgCGG-GCGCuGAccuGCGCUg -3' miRNA: 3'- -GAAACUGgGCCuUGCGcCUuu-UGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 5480 | 0.66 | 0.897865 |
Target: 5'- --gUGGCCCuuGGCGCGcagcuugcccuuggaGGAGGCGCCg -3' miRNA: 3'- gaaACUGGGccUUGCGC---------------CUUUUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 26223 | 0.66 | 0.894879 |
Target: 5'- ---cGAUCCGGucGAUGCGGAcu-CGCUc -3' miRNA: 3'- gaaaCUGGGCC--UUGCGCCUuuuGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 12553 | 0.67 | 0.887203 |
Target: 5'- ---aGACCUGGcugAACGaGGA--GCACCg -3' miRNA: 3'- gaaaCUGGGCC---UUGCgCCUuuUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 26684 | 0.67 | 0.887203 |
Target: 5'- ---aGGCCaUGGAGCGCcuugaggccuuGGggGAUAUCa -3' miRNA: 3'- gaaaCUGG-GCCUUGCG-----------CCuuUUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 7990 | 0.67 | 0.887203 |
Target: 5'- ------aCCGGAGuCGCGGGcaAAGCACUu -3' miRNA: 3'- gaaacugGGCCUU-GCGCCU--UUUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 3743 | 0.67 | 0.876787 |
Target: 5'- -cUUGACCUacgagaccgugucuGGAACGCcguuGGAGACugCa -3' miRNA: 3'- gaAACUGGG--------------CCUUGCGc---CUUUUGugG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 30614 | 0.67 | 0.870977 |
Target: 5'- --aUGACCCuGGccCGUcuGGAcGAGCACCg -3' miRNA: 3'- gaaACUGGG-CCuuGCG--CCU-UUUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 30546 | 0.67 | 0.869292 |
Target: 5'- ----aGCCCGGAGCGCaccccuuCACCg -3' miRNA: 3'- gaaacUGGGCCUUGCGccuuuu-GUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 12184 | 0.67 | 0.853634 |
Target: 5'- ---gGACCUGGGcugGCGGu-GGCACCc -3' miRNA: 3'- gaaaCUGGGCCUug-CGCCuuUUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 29908 | 0.67 | 0.853634 |
Target: 5'- ----cACCCGGucACGUGGccuACACCu -3' miRNA: 3'- gaaacUGGGCCu-UGCGCCuuuUGUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 362 | 0.68 | 0.844565 |
Target: 5'- aCUUUGACCguuuACGUGGAGAcuCGCCc -3' miRNA: 3'- -GAAACUGGgccuUGCGCCUUUu-GUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 15045 | 0.68 | 0.844565 |
Target: 5'- ---aGGCCCaGc-CGCGGAAAGcCGCCc -3' miRNA: 3'- gaaaCUGGGcCuuGCGCCUUUU-GUGG- -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 68 | 0.68 | 0.835244 |
Target: 5'- --gUGGCgCGGGGCGUGGGAAcgGgGCg -3' miRNA: 3'- gaaACUGgGCCUUGCGCCUUU--UgUGg -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 68 | 0.68 | 0.835244 |
Target: 5'- --gUGGCgCGGGGCGUGGGAAcgGgGCg -3' miRNA: 3'- gaaACUGgGCCUUGCGCCUUU--UgUGg -5' |
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215 | 3' | -49.5 | AC_000008.1 | + | 18891 | 0.68 | 0.825682 |
Target: 5'- gCUUUaGGCCgcgcuuuucuuCGGAcggugcaaGCGCGGgcAGCACCu -3' miRNA: 3'- -GAAA-CUGG-----------GCCU--------UGCGCCuuUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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