miRNA: 3'- aaGUCaa-GCaaGACCaGGGCCAa--UCG- -5'
miRNA: 3'- aAGUCAaGCAAGACCa---GGGCCa-AUCg -5'
miRNA: 3'- aaGUCAAGCAAg--ACCAgGGCCaa--UCG- -5'
miRNA: 3'- aAgUCAaGCAAGACCaGGGCCaa---UCG- -5'
miRNA: 3'- -AAGUCAAGCAAGACCAGGGCCAAUCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21733 | 5' | -51.3 | NC_004813.1 | + | 48798 | 0.66 | 0.913634 |
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Target: 5'- -aCAGgugCGg-CUGG-CCCGGUgggGGCa -3' miRNA: 3'- aaGUCaa-GCaaGACCaGGGCCAa--UCG- -5' |
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| 21733 | 5' | -51.3 | NC_004813.1 | + | 39231 | 0.66 | 0.892772 |
|
Target: 5'- aUCAGUaUGUUCUGGcgaaCCCGGgcUGGa -3' miRNA: 3'- aAGUCAaGCAAGACCa---GGGCCa-AUCg -5' |
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| 21733 | 5' | -51.3 | NC_004813.1 | + | 12951 | 0.67 | 0.877455 |
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Target: 5'- -gCAGUUUGUUgcaUGGUgCCGGgagaAGCa -3' miRNA: 3'- aaGUCAAGCAAg--ACCAgGGCCaa--UCG- -5' |
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| 21733 | 5' | -51.3 | NC_004813.1 | + | 27553 | 0.69 | 0.744203 |
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Target: 5'- cUgAGUgCGUUCUGGaCCCGGgaggaGGCu -3' miRNA: 3'- aAgUCAaGCAAGACCaGGGCCaa---UCG- -5' |
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| 21733 | 5' | -51.3 | NC_004813.1 | + | 12888 | 1.09 | 0.00245 |
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Target: 5'- aUUCAGUUCGUUCUGGUCCCGGUUAGCc -3' miRNA: 3'- -AAGUCAAGCAAGACCAGGGCCAAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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