Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21734 | 5' | -52.3 | NC_004813.1 | + | 56360 | 0.72 | 0.531926 |
Target: 5'- uUACGC-UGCCGAGCAUuuugguaaGACAugGgCCg -3' miRNA: 3'- cAUGCGuGCGGUUCGUA--------CUGUugC-GG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 48408 | 0.67 | 0.832601 |
Target: 5'- -aAUGCA-GCCAGGgAUG-CAACGaCCg -3' miRNA: 3'- caUGCGUgCGGUUCgUACuGUUGC-GG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 48324 | 0.66 | 0.850449 |
Target: 5'- --cCGCcUGCCGGGC-UGACuccGAUGCCu -3' miRNA: 3'- cauGCGuGCGGUUCGuACUG---UUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 45584 | 0.68 | 0.780253 |
Target: 5'- -aGCGCACGCCcuucaccacccgcGGCGUGuccCAuuucaccuGCGCCa -3' miRNA: 3'- caUGCGUGCGGu------------UCGUACu--GU--------UGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 44676 | 0.75 | 0.35452 |
Target: 5'- -aGCGCAuCGCCGAGCAgaaaucacUGAgAACGUCc -3' miRNA: 3'- caUGCGU-GCGGUUCGU--------ACUgUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 42118 | 0.72 | 0.553811 |
Target: 5'- -gGCGCAcacCGCCGGGCAU----GCGCCa -3' miRNA: 3'- caUGCGU---GCGGUUCGUAcuguUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 42038 | 0.72 | 0.553811 |
Target: 5'- -gGCGCAUGCCcGGCGgUGu--GCGCCu -3' miRNA: 3'- caUGCGUGCGGuUCGU-ACuguUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 38340 | 0.66 | 0.875407 |
Target: 5'- cGUGCaCugGCUgcGGGUAUGGgCGugGCCc -3' miRNA: 3'- -CAUGcGugCGG--UUCGUACU-GUugCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 37846 | 0.7 | 0.642123 |
Target: 5'- -gGCGgACGUCAGGCugcUGcggcagccagggaGCAGCGCCg -3' miRNA: 3'- caUGCgUGCGGUUCGu--AC-------------UGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 32592 | 0.72 | 0.521101 |
Target: 5'- uGUAUGC-CGCCGGGC-UGGCGgccACGCa -3' miRNA: 3'- -CAUGCGuGCGGUUCGuACUGU---UGCGg -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 31509 | 0.67 | 0.794347 |
Target: 5'- -aGCGCAUuuUuacGCAUgGACAGCGCCa -3' miRNA: 3'- caUGCGUGcgGuu-CGUA-CUGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 31022 | 0.68 | 0.763762 |
Target: 5'- cGUGUGCACGcCCAGGUGUcgGGCggUGCUg -3' miRNA: 3'- -CAUGCGUGC-GGUUCGUA--CUGuuGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 29359 | 0.71 | 0.575955 |
Target: 5'- -cAUGCGC-CCAGGCAUGGCcccaGCCc -3' miRNA: 3'- caUGCGUGcGGUUCGUACUGuug-CGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 29241 | 0.66 | 0.86734 |
Target: 5'- -aGCGCGCugcaggauaucGuCCAGGC-UGAC-GCGCCu -3' miRNA: 3'- caUGCGUG-----------C-GGUUCGuACUGuUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 28426 | 0.68 | 0.753268 |
Target: 5'- -cACGCuggcUGCCGGGaucaugAUGACGGCGCUg -3' miRNA: 3'- caUGCGu---GCGGUUCg-----UACUGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 28077 | 0.68 | 0.784313 |
Target: 5'- gGUGCGUgaacuGCGCCAGcuuauaagccGCAUc-CAGCGCCa -3' miRNA: 3'- -CAUGCG-----UGCGGUU----------CGUAcuGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 27620 | 0.68 | 0.753268 |
Target: 5'- -aACGCACucaGCCGGGUAUGGuuCAGC-CCa -3' miRNA: 3'- caUGCGUG---CGGUUCGUACU--GUUGcGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 24522 | 0.7 | 0.631998 |
Target: 5'- aUGCGaccagguuaACGCCAuugAGCGUGAUAagGCGCUg -3' miRNA: 3'- cAUGCg--------UGCGGU---UCGUACUGU--UGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 23192 | 0.66 | 0.86734 |
Target: 5'- aUAC-CGgGCCAuGCGUGACGucugGCGCa -3' miRNA: 3'- cAUGcGUgCGGUuCGUACUGU----UGCGg -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 22311 | 0.66 | 0.850449 |
Target: 5'- -cGCGUuuaGCGCCAcucuucccGCAUGGCGcaaaauCGCCg -3' miRNA: 3'- caUGCG---UGCGGUu-------CGUACUGUu-----GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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