Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21734 | 5' | -52.3 | NC_004813.1 | + | 14894 | 1.13 | 0.001091 |
Target: 5'- gGUACGCACGCCAAGCAUGACAACGCCa -3' miRNA: 3'- -CAUGCGUGCGGUUCGUACUGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 14685 | 0.66 | 0.86734 |
Target: 5'- -gAUGUuucGCGUCAGGUgcuAUGGCAugGUCa -3' miRNA: 3'- caUGCG---UGCGGUUCG---UACUGUugCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 13493 | 0.66 | 0.84164 |
Target: 5'- uUAUGCugguuacuguuGCGCCuguuAGCGcGGCAACGUCc -3' miRNA: 3'- cAUGCG-----------UGCGGu---UCGUaCUGUUGCGG- -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 13162 | 0.71 | 0.587103 |
Target: 5'- -gACGCAaggcUGCCGAugaacugaccuGUAUGACAGCGCg -3' miRNA: 3'- caUGCGU----GCGGUU-----------CGUACUGUUGCGg -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 13127 | 0.67 | 0.813873 |
Target: 5'- -cAUGCGgGCCAAuGCGcUGACuGACGCg -3' miRNA: 3'- caUGCGUgCGGUU-CGU-ACUG-UUGCGg -5' |
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21734 | 5' | -52.3 | NC_004813.1 | + | 3197 | 0.67 | 0.793351 |
Target: 5'- -cAUGCAggcUGCCAuuaucgaauggcaGGCAacUGGCGACGCUa -3' miRNA: 3'- caUGCGU---GCGGU-------------UCGU--ACUGUUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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