Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21734 | 5' | -52.3 | NC_004813.1 | + | 42118 | 0.72 | 0.553811 |
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Target: 5'- -gGCGCAcacCGCCGGGCAU----GCGCCa -3' miRNA: 3'- caUGCGU---GCGGUUCGUAcuguUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 44676 | 0.75 | 0.35452 |
|
Target: 5'- -aGCGCAuCGCCGAGCAgaaaucacUGAgAACGUCc -3' miRNA: 3'- caUGCGU-GCGGUUCGU--------ACUgUUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 45584 | 0.68 | 0.780253 |
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Target: 5'- -aGCGCACGCCcuucaccacccgcGGCGUGuccCAuuucaccuGCGCCa -3' miRNA: 3'- caUGCGUGCGGu------------UCGUACu--GU--------UGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 48324 | 0.66 | 0.850449 |
|
Target: 5'- --cCGCcUGCCGGGC-UGACuccGAUGCCu -3' miRNA: 3'- cauGCGuGCGGUUCGuACUG---UUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 48408 | 0.67 | 0.832601 |
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Target: 5'- -aAUGCA-GCCAGGgAUG-CAACGaCCg -3' miRNA: 3'- caUGCGUgCGGUUCgUACuGUUGC-GG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 56360 | 0.72 | 0.531926 |
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Target: 5'- uUACGC-UGCCGAGCAUuuugguaaGACAugGgCCg -3' miRNA: 3'- cAUGCGuGCGGUUCGUA--------CUGUugC-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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