Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21734 | 5' | -52.3 | NC_004813.1 | + | 31509 | 0.67 | 0.794347 |
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Target: 5'- -aGCGCAUuuUuacGCAUgGACAGCGCCa -3' miRNA: 3'- caUGCGUGcgGuu-CGUA-CUGUUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 13493 | 0.66 | 0.84164 |
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Target: 5'- uUAUGCugguuacuguuGCGCCuguuAGCGcGGCAACGUCc -3' miRNA: 3'- cAUGCG-----------UGCGGu---UCGUaCUGUUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 22311 | 0.66 | 0.850449 |
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Target: 5'- -cGCGUuuaGCGCCAcucuucccGCAUGGCGcaaaauCGCCg -3' miRNA: 3'- caUGCG---UGCGGUu-------CGUACUGUu-----GCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 48324 | 0.66 | 0.850449 |
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Target: 5'- --cCGCcUGCCGGGC-UGACuccGAUGCCu -3' miRNA: 3'- cauGCGuGCGGUUCGuACUG---UUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 29241 | 0.66 | 0.86734 |
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Target: 5'- -aGCGCGCugcaggauaucGuCCAGGC-UGAC-GCGCCu -3' miRNA: 3'- caUGCGUG-----------C-GGUUCGuACUGuUGCGG- -5' |
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| 21734 | 5' | -52.3 | NC_004813.1 | + | 14894 | 1.13 | 0.001091 |
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Target: 5'- gGUACGCACGCCAAGCAUGACAACGCCa -3' miRNA: 3'- -CAUGCGUGCGGUUCGUACUGUUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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