Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21740 | 3' | -57.6 | NC_004813.1 | + | 48698 | 0.67 | 0.569744 |
Target: 5'- gCGGGgaGagaCCGGUcugacgggaaauGCAggUCCACAggGUCCa -3' miRNA: 3'- -GCCCgaCg--GGCCA------------CGU--AGGUGU--UAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 46384 | 0.67 | 0.538056 |
Target: 5'- gGGGCUGgCCaucaGUAUCCuCAAUCCc -3' miRNA: 3'- gCCCGACgGGcca-CGUAGGuGUUAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 45252 | 0.69 | 0.419056 |
Target: 5'- gCGGGgUGUgCGGUGaUGUCCACAcUCa -3' miRNA: 3'- -GCCCgACGgGCCAC-GUAGGUGUuAGg -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 42292 | 0.66 | 0.591125 |
Target: 5'- uGGuCUGCCgGgGUGaCAUUCACAAauUCCg -3' miRNA: 3'- gCCcGACGGgC-CAC-GUAGGUGUU--AGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 38646 | 0.68 | 0.505973 |
Target: 5'- gGGGUUGCCCGGUaCuUUgGCAAuauggccUCCg -3' miRNA: 3'- gCCCGACGGGCCAcGuAGgUGUU-------AGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 35855 | 0.67 | 0.538056 |
Target: 5'- cCGGGCgcacaaacggGUgCGGUGgcaUAUUCACGGUCCc -3' miRNA: 3'- -GCCCGa---------CGgGCCAC---GUAGGUGUUAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 35437 | 0.69 | 0.400793 |
Target: 5'- aCGGGCaGCCCGacaaCGUUCAgGAUCCg -3' miRNA: 3'- -GCCCGaCGGGCcac-GUAGGUgUUAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 35368 | 0.68 | 0.506995 |
Target: 5'- uCGGGCUGCCCG-UGCcggaUGCGA-CCg -3' miRNA: 3'- -GCCCGACGGGCcACGuag-GUGUUaGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 34944 | 0.67 | 0.569744 |
Target: 5'- gGGGCUGCcaugcgacugaCCGGgaUGCuguaCCGgAAUCCg -3' miRNA: 3'- gCCCGACG-----------GGCC--ACGua--GGUgUUAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 32578 | 0.68 | 0.486717 |
Target: 5'- aGGGUUGCcgCCGGUGUAUgCCGCcgggCUg -3' miRNA: 3'- gCCCGACG--GGCCACGUA-GGUGuua-GG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 32519 | 0.71 | 0.349151 |
Target: 5'- aCGGuGcCUGCCCGGgaaGUgAUCCACGA-CCg -3' miRNA: 3'- -GCC-C-GACGGGCCa--CG-UAGGUGUUaGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 28352 | 1.12 | 0.000387 |
Target: 5'- uCGGGCUGCCCGGUGCAUCCACAAUCCg -3' miRNA: 3'- -GCCCGACGGGCCACGUAGGUGUUAGG- -5' |
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21740 | 3' | -57.6 | NC_004813.1 | + | 27534 | 0.67 | 0.526585 |
Target: 5'- uGGGCUGaaccauaCCCGGcugagUGCGUUCugGAcCCg -3' miRNA: 3'- gCCCGAC-------GGGCC-----ACGUAGGugUUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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