Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21745 | 3' | -61.1 | NC_004813.1 | + | 21056 | 0.66 | 0.405593 |
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Target: 5'- aGUCCGGaagccugaaugacaCACGGGgacuaCAGCCCGAaauACGa -3' miRNA: 3'- -UAGGCC--------------GUGCCC-----GUCGGGCUgu-UGCa -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 44977 | 0.66 | 0.381895 |
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Target: 5'- -cCCGGCACGGugGGCCUGACccGACu- -3' miRNA: 3'- uaGGCCGUGCCcgUCGGGCUG--UUGca -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 27360 | 0.67 | 0.356669 |
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Target: 5'- -cCCGGgGCGGGUcacAGUCCGAUGAUa- -3' miRNA: 3'- uaGGCCgUGCCCG---UCGGGCUGUUGca -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 48753 | 0.67 | 0.356669 |
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Target: 5'- -aCCGGUGCGGcaGGCCCGGCAGg-- -3' miRNA: 3'- uaGGCCGUGCCcgUCGGGCUGUUgca -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 30177 | 0.67 | 0.356669 |
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Target: 5'- uAUCCGGCACGGGCuucGCCaguGuuGcCGUg -3' miRNA: 3'- -UAGGCCGUGCCCGu--CGGg--CugUuGCA- -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 40553 | 0.67 | 0.348524 |
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Target: 5'- uGUCCGGUGCGGGaggauuuCUCGGCAGCa- -3' miRNA: 3'- -UAGGCCGUGCCCguc----GGGCUGUUGca -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 28279 | 0.68 | 0.302476 |
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Target: 5'- ----uGCAcCGGGCAGCCCGACGuuCGg -3' miRNA: 3'- uaggcCGU-GCCCGUCGGGCUGUu-GCa -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 28837 | 0.68 | 0.288204 |
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Target: 5'- uUCCGG-ACGGGaggcaAGCCCGcCGAUGg -3' miRNA: 3'- uAGGCCgUGCCCg----UCGGGCuGUUGCa -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 45260 | 0.74 | 0.110004 |
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Target: 5'- gGUCCGGCGCGGGguGUgCGGUGAUGUc -3' miRNA: 3'- -UAGGCCGUGCCCguCGgGCUGUUGCA- -5' |
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| 21745 | 3' | -61.1 | NC_004813.1 | + | 35445 | 1.04 | 0.000601 |
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Target: 5'- cAUCCGGCACGGGCAGCCCGACAACGUu -3' miRNA: 3'- -UAGGCCGUGCCCGUCGGGCUGUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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