Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21749 | 5' | -54 | NC_004813.1 | + | 38660 | 1.13 | 0.000747 |
Target: 5'- cUGUCCGGCAACACCGUUCAGCAUGCCg -3' miRNA: 3'- -ACAGGCCGUUGUGGCAAGUCGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 21395 | 0.75 | 0.30385 |
Target: 5'- aGUaCGGUGACACCGggcggugCAGCAUGCa -3' miRNA: 3'- aCAgGCCGUUGUGGCaa-----GUCGUACGg -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 45491 | 0.75 | 0.319355 |
Target: 5'- cUG-CCGGagggauGACGCUGUUCAGgGUGCCu -3' miRNA: 3'- -ACaGGCCg-----UUGUGGCAAGUCgUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 31115 | 0.72 | 0.414972 |
Target: 5'- aG-CCGGCAGCACCGcccgacaccUgGGCGUGCa -3' miRNA: 3'- aCaGGCCGUUGUGGCa--------AgUCGUACGg -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 54242 | 0.71 | 0.512428 |
Target: 5'- gUGUCUGGCuuuaauccuggaguGuaGCAUUGUaCAGCAUGCCc -3' miRNA: 3'- -ACAGGCCG--------------U--UGUGGCAaGUCGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 14789 | 0.71 | 0.474002 |
Target: 5'- -uUCCGGCGccaGCCGUUgAcCAUGCCa -3' miRNA: 3'- acAGGCCGUug-UGGCAAgUcGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 22931 | 0.71 | 0.494603 |
Target: 5'- cUGaCCGGUGACugCG-UCAGCgugaacgguGUGCCa -3' miRNA: 3'- -ACaGGCCGUUGugGCaAGUCG---------UACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 30087 | 0.71 | 0.515602 |
Target: 5'- -aUCaCGGCAACACUGgcgaAGCccGUGCCg -3' miRNA: 3'- acAG-GCCGUUGUGGCaag-UCG--UACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 44881 | 0.7 | 0.580487 |
Target: 5'- cGUUCGGCuGCACCaGUcgcggUCAGgccccCGUGCCg -3' miRNA: 3'- aCAGGCCGuUGUGG-CA-----AGUC-----GUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 21470 | 0.7 | 0.5586 |
Target: 5'- cGcCCGGUGuCACCGUacugCGGCGcGCCa -3' miRNA: 3'- aCaGGCCGUuGUGGCAa---GUCGUaCGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 42673 | 0.69 | 0.602547 |
Target: 5'- cUGUCUGGCGuCACCGccacuggCAcGCAucUGCCu -3' miRNA: 3'- -ACAGGCCGUuGUGGCaa-----GU-CGU--ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 30718 | 0.69 | 0.613621 |
Target: 5'- --aCCGGCuGCGCCGU--GGCG-GCCu -3' miRNA: 3'- acaGGCCGuUGUGGCAagUCGUaCGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 48657 | 0.68 | 0.691027 |
Target: 5'- gGUCCGGCAGguCCG--CAGgG-GCCg -3' miRNA: 3'- aCAGGCCGUUguGGCaaGUCgUaCGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 37095 | 0.68 | 0.6469 |
Target: 5'- aGUCUGGcCAGCGCCaGUguuuUCAGaucuuuUGCCa -3' miRNA: 3'- aCAGGCC-GUUGUGG-CA----AGUCgu----ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 27304 | 0.68 | 0.657979 |
Target: 5'- -cUCCGGCAGCGUCGggucCGGCAUaCCa -3' miRNA: 3'- acAGGCCGUUGUGGCaa--GUCGUAcGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 17996 | 0.68 | 0.669035 |
Target: 5'- aUGUCCGGCAuccCACUGagCAGaCGUGa- -3' miRNA: 3'- -ACAGGCCGUu--GUGGCaaGUC-GUACgg -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 38782 | 0.67 | 0.744799 |
Target: 5'- ----gGGCAGCcguguACCGUgcUCAGCAgGCCg -3' miRNA: 3'- acaggCCGUUG-----UGGCA--AGUCGUaCGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 27622 | 0.67 | 0.701944 |
Target: 5'- aUGUCCGGgaaaaacaaAACAgCG-UCAGCcauUGCCg -3' miRNA: 3'- -ACAGGCCg--------UUGUgGCaAGUCGu--ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 46347 | 0.67 | 0.723556 |
Target: 5'- --aCCGGCAACAUag---GGUAUGCCa -3' miRNA: 3'- acaGGCCGUUGUGgcaagUCGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 30349 | 0.67 | 0.755252 |
Target: 5'- cGUCCGGCAACcggaaACCGguggugaaaaUC-GCAucuucUGCCg -3' miRNA: 3'- aCAGGCCGUUG-----UGGCa---------AGuCGU-----ACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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