Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21749 | 5' | -54 | NC_004813.1 | + | 30349 | 0.67 | 0.755252 |
Target: 5'- cGUCCGGCAACcggaaACCGguggugaaaaUC-GCAucuucUGCCg -3' miRNA: 3'- aCAGGCCGUUG-----UGGCa---------AGuCGU-----ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 45301 | 0.67 | 0.755252 |
Target: 5'- cGUCCGGgaaAACACgGa-CGGCAccuuUGCCa -3' miRNA: 3'- aCAGGCCg--UUGUGgCaaGUCGU----ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 40787 | 0.66 | 0.765578 |
Target: 5'- gGUCCGGCcuCcCCcaUCAGCccgGCCc -3' miRNA: 3'- aCAGGCCGuuGuGGcaAGUCGua-CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 45566 | 0.66 | 0.773739 |
Target: 5'- cGUCUGGUgaccaccgccaucAccgcggauacagaGCACCGUUuCAGCGgucUGCCg -3' miRNA: 3'- aCAGGCCG-------------U-------------UGUGGCAA-GUCGU---ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 29995 | 0.66 | 0.774752 |
Target: 5'- aGUuuGGCAguGCGcucCCGUUucucuggCAGCAUGaCCa -3' miRNA: 3'- aCAggCCGU--UGU---GGCAA-------GUCGUAC-GG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 33166 | 0.66 | 0.775764 |
Target: 5'- cUGgCCGGCGgugauGCACUGUaucuUCAGCA-GCa -3' miRNA: 3'- -ACaGGCCGU-----UGUGGCA----AGUCGUaCGg -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 45200 | 0.66 | 0.785799 |
Target: 5'- gGUUCaccguGGCGGCACCGgucucCGGCAgUGUCa -3' miRNA: 3'- aCAGG-----CCGUUGUGGCaa---GUCGU-ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 21981 | 0.66 | 0.785799 |
Target: 5'- -aUCCaGGgAACACCGc-CAGCAgaGCCa -3' miRNA: 3'- acAGG-CCgUUGUGGCaaGUCGUa-CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 28729 | 0.66 | 0.80441 |
Target: 5'- --aCCGG--ACACCGUauucggaUCGGC-UGCCa -3' miRNA: 3'- acaGGCCguUGUGGCA-------AGUCGuACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 14758 | 0.66 | 0.805372 |
Target: 5'- --aCCugacGCGaaACAUCGUUCAGCAUcgGCCa -3' miRNA: 3'- acaGGc---CGU--UGUGGCAAGUCGUA--CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 5695 | 0.66 | 0.805372 |
Target: 5'- aUGUUaCGGCAuaaaGCCaugaUUCAGUGUGCCc -3' miRNA: 3'- -ACAG-GCCGUug--UGGc---AAGUCGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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