Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21749 | 5' | -54 | NC_004813.1 | + | 45491 | 0.75 | 0.319355 |
Target: 5'- cUG-CCGGagggauGACGCUGUUCAGgGUGCCu -3' miRNA: 3'- -ACaGGCCg-----UUGUGGCAAGUCgUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 27304 | 0.68 | 0.657979 |
Target: 5'- -cUCCGGCAGCGUCGggucCGGCAUaCCa -3' miRNA: 3'- acAGGCCGUUGUGGCaa--GUCGUAcGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 17996 | 0.68 | 0.669035 |
Target: 5'- aUGUCCGGCAuccCACUGagCAGaCGUGa- -3' miRNA: 3'- -ACAGGCCGUu--GUGGCaaGUC-GUACgg -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 46347 | 0.67 | 0.723556 |
Target: 5'- --aCCGGCAACAUag---GGUAUGCCa -3' miRNA: 3'- acaGGCCGUUGUGgcaagUCGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 40787 | 0.66 | 0.765578 |
Target: 5'- gGUCCGGCcuCcCCcaUCAGCccgGCCc -3' miRNA: 3'- aCAGGCCGuuGuGGcaAGUCGua-CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 45200 | 0.66 | 0.785799 |
Target: 5'- gGUUCaccguGGCGGCACCGgucucCGGCAgUGUCa -3' miRNA: 3'- aCAGG-----CCGUUGUGGCaa---GUCGU-ACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 21981 | 0.66 | 0.785799 |
Target: 5'- -aUCCaGGgAACACCGc-CAGCAgaGCCa -3' miRNA: 3'- acAGG-CCgUUGUGGCaaGUCGUa-CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 28729 | 0.66 | 0.80441 |
Target: 5'- --aCCGG--ACACCGUauucggaUCGGC-UGCCa -3' miRNA: 3'- acaGGCCguUGUGGCA-------AGUCGuACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 5695 | 0.66 | 0.805372 |
Target: 5'- aUGUUaCGGCAuaaaGCCaugaUUCAGUGUGCCc -3' miRNA: 3'- -ACAG-GCCGUug--UGGc---AAGUCGUACGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 14758 | 0.66 | 0.805372 |
Target: 5'- --aCCugacGCGaaACAUCGUUCAGCAUcgGCCa -3' miRNA: 3'- acaGGc---CGU--UGUGGCAAGUCGUA--CGG- -5' |
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21749 | 5' | -54 | NC_004813.1 | + | 38660 | 1.13 | 0.000747 |
Target: 5'- cUGUCCGGCAACACCGUUCAGCAUGCCg -3' miRNA: 3'- -ACAGGCCGUUGUGGCAAGUCGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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