Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21759 | 3' | -51.5 | NC_004813.1 | + | 36107 | 0.66 | 0.913287 |
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Target: 5'- gGACGCCCGUGAagccauaaugcugcUGGccccgggauCCGUCAGUGa -3' miRNA: 3'- gCUGUGGGUGCU--------------ACUuu-------GGCAGUCAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 35939 | 0.66 | 0.908712 |
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Target: 5'- uGugGuCCcccugCugGAUGGcaGGCCGUCGGUGg -3' miRNA: 3'- gCugU-GG-----GugCUACU--UUGGCAGUCAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 21384 | 0.66 | 0.901937 |
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Target: 5'- uGGCGCgCCgcaguACGGUGAcACCGggCGGUGc -3' miRNA: 3'- gCUGUG-GG-----UGCUACUuUGGCa-GUCAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 52961 | 0.67 | 0.88756 |
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Target: 5'- aGGCGaCCGCGu--AAACCGUCAGUu -3' miRNA: 3'- gCUGUgGGUGCuacUUUGGCAGUCAc -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 15259 | 0.67 | 0.863998 |
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Target: 5'- --cCACCCACGGccUGAuauuGCUGUaCGGUGg -3' miRNA: 3'- gcuGUGGGUGCU--ACUu---UGGCA-GUCAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 29283 | 0.68 | 0.847034 |
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Target: 5'- gGGCcauGCCUggGCGcAUGAAACCG-CGGUGg -3' miRNA: 3'- gCUG---UGGG--UGC-UACUUUGGCaGUCAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 30568 | 0.68 | 0.819871 |
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Target: 5'- aCGACAacaCCGCaGcgGAAAuCCGUCAgGUGg -3' miRNA: 3'- -GCUGUg--GGUG-CuaCUUU-GGCAGU-CAC- -5' |
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| 21759 | 3' | -51.5 | NC_004813.1 | + | 47761 | 1.08 | 0.003191 |
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Target: 5'- cCGACACCCACGAUGAAACCGUCAGUGc -3' miRNA: 3'- -GCUGUGGGUGCUACUUUGGCAGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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