Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21832 | 3' | -50.9 | NC_004831.2 | + | 17190 | 0.66 | 0.890087 |
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Target: 5'- aACCUGCU-GGUUGAUGcUugAaugCAACCu -3' miRNA: 3'- -UGGACGGcCCAGCUAU-AugUa--GUUGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 20544 | 0.68 | 0.780784 |
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Target: 5'- -gUUGCUGGGUCGAaUGUACGUagcGCUu -3' miRNA: 3'- ugGACGGCCCAGCU-AUAUGUAgu-UGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 110 | 0.68 | 0.759675 |
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Target: 5'- gGCCUGCCGGaGcgagCGAUAgggacGCGUguaGGCCg -3' miRNA: 3'- -UGGACGGCC-Ca---GCUAUa----UGUAg--UUGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 43662 | 0.68 | 0.759675 |
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Target: 5'- gGCCUGCCGGaGcgagCGAUAgggacGCGUguaGGCCg -3' miRNA: 3'- -UGGACGGCC-Ca---GCUAUa----UGUAg--UUGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 36968 | 0.7 | 0.670587 |
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Target: 5'- gGCCUauucaGCguaGGUUGAUGUAUAUCGACCc -3' miRNA: 3'- -UGGA-----CGgc-CCAGCUAUAUGUAGUUGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 33029 | 0.71 | 0.624684 |
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Target: 5'- -aCUGCCGGGUCGGgaacccCAU-AGCCg -3' miRNA: 3'- ugGACGGCCCAGCUauau--GUAgUUGG- -5' |
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| 21832 | 3' | -50.9 | NC_004831.2 | + | 37052 | 1.12 | 0.001342 |
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Target: 5'- cACCUGCCGGGUCGAUAUACAUCAACCu -3' miRNA: 3'- -UGGACGGCCCAGCUAUAUGUAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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