Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21834 | 5' | -54.6 | NC_004831.2 | + | 31109 | 0.66 | 0.662438 |
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Target: 5'- cGCAGCAUuggaAGCcauccuaGCCAgCGGUGaUUACCa -3' miRNA: 3'- -CGUCGUA----UCG-------UGGU-GCCGCgAAUGGa -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 22734 | 0.66 | 0.652223 |
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Target: 5'- cGCAGCcggAGacugACC-CGGUGCUaaUACCUg -3' miRNA: 3'- -CGUCGua-UCg---UGGuGCCGCGA--AUGGA- -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 23131 | 0.67 | 0.581845 |
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Target: 5'- cGCAGUacguugaaAUAGCGCCACGGCaucaggauaGCCa -3' miRNA: 3'- -CGUCG--------UAUCGUGGUGCCGcgaa-----UGGa -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 17617 | 0.69 | 0.473731 |
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Target: 5'- -aGGCAUcucgauaAGCugCACGGCGUacaacguacUUACCa -3' miRNA: 3'- cgUCGUA-------UCGugGUGCCGCG---------AAUGGa -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 31629 | 0.7 | 0.424015 |
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Target: 5'- aGCAGCAgcAGCuucuGCCACGGCaucuaccuuCUUGCCUu -3' miRNA: 3'- -CGUCGUa-UCG----UGGUGCCGc--------GAAUGGA- -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 2488 | 0.7 | 0.39516 |
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Target: 5'- -aAGCAcauuUAGaCACCAuCGGCGCUU-CCUa -3' miRNA: 3'- cgUCGU----AUC-GUGGU-GCCGCGAAuGGA- -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 5076 | 0.73 | 0.28562 |
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Target: 5'- -aAGCAUAGCACUAUaGUGCUgaaUGCCUg -3' miRNA: 3'- cgUCGUAUCGUGGUGcCGCGA---AUGGA- -5' |
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| 21834 | 5' | -54.6 | NC_004831.2 | + | 38125 | 1.1 | 0.000575 |
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Target: 5'- aGCAGCAUAGCACCACGGCGCUUACCUg -3' miRNA: 3'- -CGUCGUAUCGUGGUGCCGCGAAUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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