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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21917 | 3' | -60.1 | NC_004913.1 | + | 17111 | 0.69 | 0.312525 |
|
Target: 5'- cCGCGuGGCAAGGCuGGCUg--ACCUUg -3' miRNA: 3'- -GUGC-CCGUUCCGcCCGGacaUGGAGg -5' |
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| 21917 | 3' | -60.1 | NC_004913.1 | + | 21213 | 1.11 | 0.000255 |
|
Target: 5'- cCACGGGCAAGGCGGGCCUGUACCUCCg -3' miRNA: 3'- -GUGCCCGUUCCGCCCGGACAUGGAGG- -5' |
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| 21917 | 3' | -60.1 | NC_004913.1 | + | 21313 | 0.66 | 0.483797 |
|
Target: 5'- aGCGGGC-AGGCGuGGacgcucaguuaacuCCUGUGCUgucggaCCg -3' miRNA: 3'- gUGCCCGuUCCGC-CC--------------GGACAUGGa-----GG- -5' |
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| 21917 | 3' | -60.1 | NC_004913.1 | + | 23542 | 0.67 | 0.423875 |
|
Target: 5'- cCACGGGCAaccgcuucaaccugcGGGacCGGGCUgccuuugGCUUCCg -3' miRNA: 3'- -GUGCCCGU---------------UCC--GCCCGGaca----UGGAGG- -5' |
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| 21917 | 3' | -60.1 | NC_004913.1 | + | 59208 | 0.68 | 0.367783 |
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Target: 5'- aUACGGGCAAuauuccGGUcaaGGGCCUGUucaGCUUUg -3' miRNA: 3'- -GUGCCCGUU------CCG---CCCGGACA---UGGAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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