miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21917 3' -60.1 NC_004913.1 + 59208 0.68 0.367783
Target:  5'- aUACGGGCAAuauuccGGUcaaGGGCCUGUucaGCUUUg -3'
miRNA:   3'- -GUGCCCGUU------CCG---CCCGGACA---UGGAGg -5'
21917 3' -60.1 NC_004913.1 + 23542 0.67 0.423875
Target:  5'- cCACGGGCAaccgcuucaaccugcGGGacCGGGCUgccuuugGCUUCCg -3'
miRNA:   3'- -GUGCCCGU---------------UCC--GCCCGGaca----UGGAGG- -5'
21917 3' -60.1 NC_004913.1 + 21313 0.66 0.483797
Target:  5'- aGCGGGC-AGGCGuGGacgcucaguuaacuCCUGUGCUgucggaCCg -3'
miRNA:   3'- gUGCCCGuUCCGC-CC--------------GGACAUGGa-----GG- -5'
21917 3' -60.1 NC_004913.1 + 21213 1.11 0.000255
Target:  5'- cCACGGGCAAGGCGGGCCUGUACCUCCg -3'
miRNA:   3'- -GUGCCCGUUCCGCCCGGACAUGGAGG- -5'
21917 3' -60.1 NC_004913.1 + 17111 0.69 0.312525
Target:  5'- cCGCGuGGCAAGGCuGGCUg--ACCUUg -3'
miRNA:   3'- -GUGC-CCGUUCCGcCCGGacaUGGAGg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.