Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21922 | 3' | -50.5 | NC_004913.1 | + | 20600 | 0.67 | 0.896953 |
Target: 5'- uUCAuUCAACAACug-GCUGGCAGCuuccCGg -3' miRNA: 3'- -GGU-GGUUGUUGuaaUGGCCGUCGu---GC- -5' |
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21922 | 3' | -50.5 | NC_004913.1 | + | 11629 | 0.68 | 0.874185 |
Target: 5'- uUACCGGCAAac---CCGGCGGCAa- -3' miRNA: 3'- gGUGGUUGUUguaauGGCCGUCGUgc -5' |
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21922 | 3' | -50.5 | NC_004913.1 | + | 51276 | 0.66 | 0.949932 |
Target: 5'- uCCACCcccugaagGACGGCGUUuuaCGGC-GCACc -3' miRNA: 3'- -GGUGG--------UUGUUGUAAug-GCCGuCGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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