Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21932 | 3' | -58.5 | NC_004913.1 | + | 7394 | 0.66 | 0.574139 |
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Target: 5'- aUGAUGCUgugagggcagugaUUCUGGCgagaCCGGAuGCUGg -3' miRNA: 3'- cGCUACGG-------------AAGGCCGa---GGCCU-CGACg -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 11307 | 0.67 | 0.533308 |
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Target: 5'- uGCGGUacaaCUgacgCUGGCUCCGGuGCcauUGCa -3' miRNA: 3'- -CGCUAcg--GAa---GGCCGAGGCCuCG---ACG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 20468 | 0.67 | 0.489559 |
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Target: 5'- ----cGCCaccggaucaacagaUUCUGGUUgCGGGGCUGCg -3' miRNA: 3'- cgcuaCGG--------------AAGGCCGAgGCCUCGACG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 15482 | 0.68 | 0.443754 |
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Target: 5'- -gGGUGCCUUUCGGCUgauggCUGGAGg-GUg -3' miRNA: 3'- cgCUACGGAAGGCCGA-----GGCCUCgaCG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 7147 | 0.68 | 0.443754 |
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Target: 5'- cCGGUaucugGCUgcgUCCGGCuUUCGGAGCgacgGCa -3' miRNA: 3'- cGCUA-----CGGa--AGGCCG-AGGCCUCGa---CG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 376 | 0.69 | 0.397788 |
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Target: 5'- ----cGCUUUCCGGCacUCUGGcGCUGUa -3' miRNA: 3'- cgcuaCGGAAGGCCG--AGGCCuCGACG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 57803 | 0.69 | 0.396014 |
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Target: 5'- aGCGGUGUCUgccagucgggggCCGGUUgcauuauccacgCCGGAGgUGCc -3' miRNA: 3'- -CGCUACGGAa-----------GGCCGA------------GGCCUCgACG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 21475 | 0.75 | 0.1719 |
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Target: 5'- aGCGgcGUUUUCCGGUgCCGGAGCuUGUc -3' miRNA: 3'- -CGCuaCGGAAGGCCGaGGCCUCG-ACG- -5' |
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| 21932 | 3' | -58.5 | NC_004913.1 | + | 55549 | 1.12 | 0.000313 |
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Target: 5'- cGCGAUGCCUUCCGGCUCCGGAGCUGCc -3' miRNA: 3'- -CGCUACGGAAGGCCGAGGCCUCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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