Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2194 | 3' | -55.6 | NC_001396.1 | + | 256 | 0.66 | 0.183741 |
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Target: 5'- -cCGAUGAaGUAaGCgCCGCCCACCAu -3' miRNA: 3'- cuGCUGUUgCAUgUGgGGUGGGUGGU- -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 5110 | 0.66 | 0.178057 |
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Target: 5'- uACGAUcGCGUAgACCUgACCgGCCc -3' miRNA: 3'- cUGCUGuUGCAUgUGGGgUGGgUGGu -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 1043 | 0.67 | 0.133582 |
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Target: 5'- uGAUGccgcacCGugGUACACCCC-UCCGCCu -3' miRNA: 3'- -CUGCu-----GUugCAUGUGGGGuGGGUGGu -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 3115 | 0.68 | 0.129325 |
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Target: 5'- --aGACGGCGUaccACGCgCCGCCaGCCAg -3' miRNA: 3'- cugCUGUUGCA---UGUGgGGUGGgUGGU- -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 3111 | 0.69 | 0.100864 |
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Target: 5'- cGCGACAccAUGUucgccaagaaagcagACGCUgCGCCCGCCAa -3' miRNA: 3'- cUGCUGU--UGCA---------------UGUGGgGUGGGUGGU- -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 3557 | 0.69 | 0.09631 |
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Target: 5'- gGACGACGAC--ACuGCCCCGCUCugCu -3' miRNA: 3'- -CUGCUGUUGcaUG-UGGGGUGGGugGu -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 1662 | 0.71 | 0.064539 |
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Target: 5'- aGCGGCAauuGCG-AUGCCCCACCUAUCu -3' miRNA: 3'- cUGCUGU---UGCaUGUGGGGUGGGUGGu -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 6316 | 0.75 | 0.032731 |
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Target: 5'- gGAUGGCAGCGUcgaacucacgcACGCUCgUACCCACCAc -3' miRNA: 3'- -CUGCUGUUGCA-----------UGUGGG-GUGGGUGGU- -5' |
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| 2194 | 3' | -55.6 | NC_001396.1 | + | 3604 | 1.08 | 5.5e-05 |
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Target: 5'- aGACGACAACGUACACCCCACCCACCAc -3' miRNA: 3'- -CUGCUGUUGCAUGUGGGGUGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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