Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21964 | 3' | -54.4 | NC_004914.1 | + | 32108 | 0.67 | 0.699794 |
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Target: 5'- gGGCAcucCAGCGCCAGCAuGGuCUACa- -3' miRNA: 3'- gUCGUu--GUCGUGGUUGU-CCuGGUGgu -5' |
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| 21964 | 3' | -54.4 | NC_004914.1 | + | 20366 | 0.67 | 0.710697 |
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Target: 5'- cCGGCAACcGCACCGAauGGAgCCcCCAu -3' miRNA: 3'- -GUCGUUGuCGUGGUUguCCU-GGuGGU- -5' |
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| 21964 | 3' | -54.4 | NC_004914.1 | + | 19683 | 0.67 | 0.721521 |
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Target: 5'- uCAGguACAagcGCACCAAuCAGGuuuacacccuucGCCACCc -3' miRNA: 3'- -GUCguUGU---CGUGGUU-GUCC------------UGGUGGu -5' |
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| 21964 | 3' | -54.4 | NC_004914.1 | + | 16071 | 0.66 | 0.752354 |
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Target: 5'- uCAGCAcugagcacagaacGCAGCACCAAaaa-GCCAUCAg -3' miRNA: 3'- -GUCGU-------------UGUCGUGGUUguccUGGUGGU- -5' |
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| 21964 | 3' | -54.4 | NC_004914.1 | + | 60335 | 0.66 | 0.774039 |
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Target: 5'- cCAGCcACAGCGCaCcAUAGccaACCGCCAg -3' miRNA: 3'- -GUCGuUGUCGUG-GuUGUCc--UGGUGGU- -5' |
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| 21964 | 3' | -54.4 | NC_004914.1 | + | 17713 | 1.09 | 0.001244 |
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Target: 5'- cCAGCAACAGCACCAACAGGACCACCAa -3' miRNA: 3'- -GUCGUUGUCGUGGUUGUCCUGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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