Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22030 | 3' | -52.5 | NC_004927.1 | + | 23675 | 0.66 | 0.930881 |
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Target: 5'- aGGGAugGGucacGAACAGGcCGUC-GCc-- -3' miRNA: 3'- -CCCUugCC----UUUGUCCaGCAGuCGcuc -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 30611 | 0.66 | 0.919448 |
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Target: 5'- --cGACGGAGAC-GGUCugcuUUAGCGAGa -3' miRNA: 3'- cccUUGCCUUUGuCCAGc---AGUCGCUC- -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 73055 | 0.66 | 0.91333 |
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Target: 5'- uGGAGuuCGGAGACGcuGGUauugaGcCAGCGAGg -3' miRNA: 3'- cCCUU--GCCUUUGU--CCAg----CaGUCGCUC- -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 57085 | 0.68 | 0.852593 |
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Target: 5'- cGGGAUGGAgugcuacgugaaggGugGGGaCGUUGGCGAGc -3' miRNA: 3'- cCCUUGCCU--------------UugUCCaGCAGUCGCUC- -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 31100 | 0.72 | 0.668078 |
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Target: 5'- gGGGAACGGAugUucGUCGUCGGgGuGg -3' miRNA: 3'- -CCCUUGCCUuuGucCAGCAGUCgCuC- -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 23119 | 0.74 | 0.517973 |
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Target: 5'- aGGGAGCGGGuuCuGGUUGuUCAGCGc- -3' miRNA: 3'- -CCCUUGCCUuuGuCCAGC-AGUCGCuc -5' |
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| 22030 | 3' | -52.5 | NC_004927.1 | + | 10552 | 1.12 | 0.002058 |
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Target: 5'- gGGGAACGGAAACAGGUCGUCAGCGAGg -3' miRNA: 3'- -CCCUUGCCUUUGUCCAGCAGUCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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