Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22039 | 3' | -48.5 | NC_004927.1 | + | 29638 | 0.66 | 0.984096 |
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Target: 5'- uAUCGGgAACcCGGuCGUCGUUCUCGa- -3' miRNA: 3'- -UAGUCgUUGaGUC-GUAGUAAGAGUgg -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 21971 | 0.66 | 0.979565 |
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Target: 5'- aAUCAccGCAucgaggucguACUCGGCGUCAccgacUUCACCa -3' miRNA: 3'- -UAGU--CGU----------UGAGUCGUAGUaa---GAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 47334 | 0.67 | 0.971064 |
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Target: 5'- uUCgAGaCGGCUCAGCAggcCGcUCUCAUCu -3' miRNA: 3'- uAG-UC-GUUGAGUCGUa--GUaAGAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 28527 | 0.68 | 0.96028 |
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Target: 5'- -cCAuCAACUCGGCAuacgucUCAggacgCUCGCCg -3' miRNA: 3'- uaGUcGUUGAGUCGU------AGUaa---GAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 48592 | 0.68 | 0.947001 |
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Target: 5'- aAUCGGCcACUCGuGguUCGggaacaUCUCACCc -3' miRNA: 3'- -UAGUCGuUGAGU-CguAGUa-----AGAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 2243 | 0.68 | 0.941993 |
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Target: 5'- uUC-GCAccACUUAGCgauGUCAcUCUCGCCg -3' miRNA: 3'- uAGuCGU--UGAGUCG---UAGUaAGAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 22928 | 0.68 | 0.941993 |
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Target: 5'- cGUCAGCucCUCggaAGCGUCGaugCUCACa -3' miRNA: 3'- -UAGUCGuuGAG---UCGUAGUaa-GAGUGg -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 4030 | 0.68 | 0.941993 |
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Target: 5'- gGUCGGCucuCUCGGC-UCGUUCgaggUCACa -3' miRNA: 3'- -UAGUCGuu-GAGUCGuAGUAAG----AGUGg -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 33510 | 0.71 | 0.867291 |
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Target: 5'- cUCGGC-GCUCAGggaGUCGUccuucuucaUCUCGCCa -3' miRNA: 3'- uAGUCGuUGAGUCg--UAGUA---------AGAGUGG- -5' |
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| 22039 | 3' | -48.5 | NC_004927.1 | + | 16615 | 1.12 | 0.003794 |
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Target: 5'- aAUCAGCAACUCAGCAUCAUUCUCACCa -3' miRNA: 3'- -UAGUCGUUGAGUCGUAGUAAGAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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