Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22043 | 5' | -57.9 | NC_004927.1 | + | 64723 | 0.66 | 0.707849 |
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Target: 5'- uGGCCGGauugaGGGAAUCGAggaaGCaaacgagaACGUCg -3' miRNA: 3'- gCUGGCCg----CCCUUAGCU----UGg-------UGCGGa -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 45979 | 0.66 | 0.676881 |
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Target: 5'- aCGACgGGCGGGug-UGAGCCu--CCUa -3' miRNA: 3'- -GCUGgCCGCCCuuaGCUUGGugcGGA- -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 53486 | 0.67 | 0.635094 |
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Target: 5'- uCGACCcucguGGCGGGggU-GAACgGCGUUUc -3' miRNA: 3'- -GCUGG-----CCGCCCuuAgCUUGgUGCGGA- -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 35169 | 0.68 | 0.58286 |
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Target: 5'- --cUCGGCGGGGccGUCguacuguccgaGAACCACGUCUu -3' miRNA: 3'- gcuGGCCGCCCU--UAG-----------CUUGGUGCGGA- -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 53756 | 0.7 | 0.462737 |
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Target: 5'- aCGACCGGCGGuGA---GAACCGCGa-- -3' miRNA: 3'- -GCUGGCCGCC-CUuagCUUGGUGCgga -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 10312 | 0.72 | 0.333225 |
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Target: 5'- aCGuCgCGGCGGGGcuUCGAcuuuCCACGCCg -3' miRNA: 3'- -GCuG-GCCGCCCUu-AGCUu---GGUGCGGa -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 21523 | 0.74 | 0.269244 |
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Target: 5'- uGACCGGCGGGAGUgaUGAcCUugGCg- -3' miRNA: 3'- gCUGGCCGCCCUUA--GCUuGGugCGga -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 20949 | 0.76 | 0.210178 |
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Target: 5'- gGACgGGC-GGAAUCGAACCGC-CCUa -3' miRNA: 3'- gCUGgCCGcCCUUAGCUUGGUGcGGA- -5' |
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| 22043 | 5' | -57.9 | NC_004927.1 | + | 19795 | 1.07 | 0.001362 |
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Target: 5'- gCGACCGGCGGGAAUCGAACCACGCCUu -3' miRNA: 3'- -GCUGGCCGCCCUUAGCUUGGUGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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