Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22059 | 3' | -55.1 | NC_004927.1 | + | 10932 | 0.66 | 0.784283 |
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Target: 5'- cAGGacUCGcCCUCCgUCUcgUCGGGGAgGGa -3' miRNA: 3'- cUCC--AGC-GGAGGaAGA--AGCUCCUgUC- -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 53357 | 0.66 | 0.774498 |
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Target: 5'- cAGGUCGaUUCUacCUUCGAGGACGa -3' miRNA: 3'- cUCCAGCgGAGGaaGAAGCUCCUGUc -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 9946 | 0.67 | 0.723665 |
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Target: 5'- --cGUCGaggaUUUCUUCUUCGAGGGCGu -3' miRNA: 3'- cucCAGCg---GAGGAAGAAGCUCCUGUc -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 49281 | 0.69 | 0.649111 |
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Target: 5'- aGGGGUCGUgggUUCUUCUaagCGAGGACGc -3' miRNA: 3'- -CUCCAGCGg--AGGAAGAa--GCUCCUGUc -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 65694 | 0.71 | 0.520129 |
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Target: 5'- cGAGGUCGCCaaaggauUCCgaaUCaUCGAcgGGGCAGa -3' miRNA: 3'- -CUCCAGCGG-------AGGa--AGaAGCU--CCUGUC- -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 14668 | 0.73 | 0.395177 |
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Target: 5'- cGAGGaagaUUGCCgcUCCgUCUUCGAGGAUGGg -3' miRNA: 3'- -CUCC----AGCGG--AGGaAGAAGCUCCUGUC- -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 42578 | 0.74 | 0.368994 |
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Target: 5'- aGAGGUCGCCUUCgauuUCcUCGAagucgaacuucaGGACAGa -3' miRNA: 3'- -CUCCAGCGGAGGa---AGaAGCU------------CCUGUC- -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 23741 | 0.74 | 0.335977 |
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Target: 5'- -cGGUCGCUUCUucgaccuuUUCUUCGAGGAUg- -3' miRNA: 3'- cuCCAGCGGAGG--------AAGAAGCUCCUGuc -5' |
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| 22059 | 3' | -55.1 | NC_004927.1 | + | 33373 | 1.08 | 0.001794 |
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Target: 5'- cGAGGUCGCCUCCUUCUUCGAGGACAGc -3' miRNA: 3'- -CUCCAGCGGAGGAAGAAGCUCCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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