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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22064 | 3' | -53.9 | NC_004927.1 | + | 52757 | 0.66 | 0.877332 |
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Target: 5'- gAGGACgCUuucaacUCGaUGGCGAccAUcCUCGGGUg -3' miRNA: 3'- -UCUUG-GA------AGC-ACCGCU--UGaGAGCCCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 53485 | 0.67 | 0.813297 |
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Target: 5'- -cGACCcUCGUGGCGGGggugaacggcguuucCgUCUCGGGa -3' miRNA: 3'- ucUUGGaAGCACCGCUU---------------G-AGAGCCCg -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 57060 | 0.67 | 0.818789 |
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Target: 5'- gGGGACgU---UGGCGAGCUaUCGGGUu -3' miRNA: 3'- -UCUUGgAagcACCGCUUGAgAGCCCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 64119 | 0.67 | 0.835721 |
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Target: 5'- gAGcGCCUUCG-GGCGAAUgaacaCUCGcuugaacGGCa -3' miRNA: 3'- -UCuUGGAAGCaCCGCUUGa----GAGC-------CCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 1150 | 0.68 | 0.780989 |
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Target: 5'- cGGGCCUaUGUGGUuuaGGGCcggUUCGGGCc -3' miRNA: 3'- uCUUGGAaGCACCG---CUUGa--GAGCCCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 45965 | 0.69 | 0.74081 |
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Target: 5'- uGAGCCUccuacaaCGUGGCGAACuucaagaacgUCUCcgaggacauucaGGGCg -3' miRNA: 3'- uCUUGGAa------GCACCGCUUG----------AGAG------------CCCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 3297 | 0.7 | 0.645261 |
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Target: 5'- cGGAGCCUUCGUauuccuccGGCu--CUCUCGuuGGCu -3' miRNA: 3'- -UCUUGGAAGCA--------CCGcuuGAGAGC--CCG- -5' |
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| 22064 | 3' | -53.9 | NC_004927.1 | + | 35400 | 1.11 | 0.001611 |
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Target: 5'- cAGAACCUUCGUGGCGAACUCUCGGGCc -3' miRNA: 3'- -UCUUGGAAGCACCGCUUGAGAGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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