miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22064 3' -53.9 NC_004927.1 + 1150 0.68 0.780989
Target:  5'- cGGGCCUaUGUGGUuuaGGGCcggUUCGGGCc -3'
miRNA:   3'- uCUUGGAaGCACCG---CUUGa--GAGCCCG- -5'
22064 3' -53.9 NC_004927.1 + 3297 0.7 0.645261
Target:  5'- cGGAGCCUUCGUauuccuccGGCu--CUCUCGuuGGCu -3'
miRNA:   3'- -UCUUGGAAGCA--------CCGcuuGAGAGC--CCG- -5'
22064 3' -53.9 NC_004927.1 + 35400 1.11 0.001611
Target:  5'- cAGAACCUUCGUGGCGAACUCUCGGGCc -3'
miRNA:   3'- -UCUUGGAAGCACCGCUUGAGAGCCCG- -5'
22064 3' -53.9 NC_004927.1 + 45965 0.69 0.74081
Target:  5'- uGAGCCUccuacaaCGUGGCGAACuucaagaacgUCUCcgaggacauucaGGGCg -3'
miRNA:   3'- uCUUGGAa------GCACCGCUUG----------AGAG------------CCCG- -5'
22064 3' -53.9 NC_004927.1 + 52757 0.66 0.877332
Target:  5'- gAGGACgCUuucaacUCGaUGGCGAccAUcCUCGGGUg -3'
miRNA:   3'- -UCUUG-GA------AGC-ACCGCU--UGaGAGCCCG- -5'
22064 3' -53.9 NC_004927.1 + 53485 0.67 0.813297
Target:  5'- -cGACCcUCGUGGCGGGggugaacggcguuucCgUCUCGGGa -3'
miRNA:   3'- ucUUGGaAGCACCGCUU---------------G-AGAGCCCg -5'
22064 3' -53.9 NC_004927.1 + 57060 0.67 0.818789
Target:  5'- gGGGACgU---UGGCGAGCUaUCGGGUu -3'
miRNA:   3'- -UCUUGgAagcACCGCUUGAgAGCCCG- -5'
22064 3' -53.9 NC_004927.1 + 64119 0.67 0.835721
Target:  5'- gAGcGCCUUCG-GGCGAAUgaacaCUCGcuugaacGGCa -3'
miRNA:   3'- -UCuUGGAAGCaCCGCUUGa----GAGC-------CCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.