Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22078 | 5' | -55.7 | NC_004927.1 | + | 47406 | 1.09 | 0.001325 |
Target: 5'- aACGCCGGGACGGACAUGAAUCAGCUCu -3' miRNA: 3'- -UGCGGCCCUGCCUGUACUUAGUCGAG- -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 26566 | 0.71 | 0.494313 |
Target: 5'- cCGuCCGGGgugguagcgGCGGAgGUGggUCGGCa- -3' miRNA: 3'- uGC-GGCCC---------UGCCUgUACuuAGUCGag -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 75542 | 0.69 | 0.599095 |
Target: 5'- -aGUCGGGGuCGGAUGgggagGggUCGGCUa -3' miRNA: 3'- ugCGGCCCU-GCCUGUa----CuuAGUCGAg -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 50395 | 0.69 | 0.599095 |
Target: 5'- cUGCCGGGAgCGGugGUGGuggugcuggcgGUCAGaCUg -3' miRNA: 3'- uGCGGCCCU-GCCugUACU-----------UAGUC-GAg -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 69245 | 0.67 | 0.69575 |
Target: 5'- cGCGCCGaGGAUGGugAgcugGuuUCGGUUg -3' miRNA: 3'- -UGCGGC-CCUGCCugUa---CuuAGUCGAg -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 65788 | 0.67 | 0.716838 |
Target: 5'- -aGCgCGGGAUGGACA--AGUCuGCUa -3' miRNA: 3'- ugCG-GCCCUGCCUGUacUUAGuCGAg -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 28811 | 0.67 | 0.727267 |
Target: 5'- gAUGCCGGGAaccguUGcGGCGUGAagaagggcGUCGGCg- -3' miRNA: 3'- -UGCGGCCCU-----GC-CUGUACU--------UAGUCGag -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 62166 | 0.66 | 0.774884 |
Target: 5'- -gGCCGuGGGaaacguuccaccCGGAUGUGAAcgaaauccggcuacUCAGCUCg -3' miRNA: 3'- ugCGGC-CCU------------GCCUGUACUU--------------AGUCGAG- -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 24531 | 0.66 | 0.78755 |
Target: 5'- aGCGCCGGGAUGuGGCGUau-UCGGa-- -3' miRNA: 3'- -UGCGGCCCUGC-CUGUAcuuAGUCgag -5' |
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22078 | 5' | -55.7 | NC_004927.1 | + | 20233 | 0.66 | 0.78755 |
Target: 5'- uCGCCgGGGACGGACuugccacgGAggucgaacacGUCGGC-Ca -3' miRNA: 3'- uGCGG-CCCUGCCUGua------CU----------UAGUCGaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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