miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22083 3' -54.6 NC_004927.1 + 71828 0.66 0.838124
Target:  5'- aAGGCCCGcguCGGuaUCUCCGGUaucacgaGGUCg -3'
miRNA:   3'- gUUUGGGCu--GUC--AGAGGCCGaa-----CCAG- -5'
22083 3' -54.6 NC_004927.1 + 7470 0.66 0.837254
Target:  5'- uCGAACCCGgcucccGCAGUCUuuacaCCGccacuuuGCUUGGUa -3'
miRNA:   3'- -GUUUGGGC------UGUCAGA-----GGC-------CGAACCAg -5'
22083 3' -54.6 NC_004927.1 + 59394 0.66 0.820326
Target:  5'- cCAAACUCGGCAcacccgagcGUCggCGGCgguaaUGGUCg -3'
miRNA:   3'- -GUUUGGGCUGU---------CAGagGCCGa----ACCAG- -5'
22083 3' -54.6 NC_004927.1 + 2180 0.67 0.782502
Target:  5'- gCGAACCuCGugAGcuaagCUCaCGGUggagUGGUCg -3'
miRNA:   3'- -GUUUGG-GCugUCa----GAG-GCCGa---ACCAG- -5'
22083 3' -54.6 NC_004927.1 + 4255 0.67 0.752506
Target:  5'- uCGAAUCCacgagaGGCAGUCUCCGGUgucccacaGGUa -3'
miRNA:   3'- -GUUUGGG------CUGUCAGAGGCCGaa------CCAg -5'
22083 3' -54.6 NC_004927.1 + 71296 0.68 0.710895
Target:  5'- gGAGCgCGACGGUUgggCgGGCUugaagauuucUGGUCa -3'
miRNA:   3'- gUUUGgGCUGUCAGa--GgCCGA----------ACCAG- -5'
22083 3' -54.6 NC_004927.1 + 74930 0.68 0.700277
Target:  5'- aAGAUgCGGCAGUaguacCUCCGGgUaaUGGUCa -3'
miRNA:   3'- gUUUGgGCUGUCA-----GAGGCCgA--ACCAG- -5'
22083 3' -54.6 NC_004927.1 + 23835 0.69 0.678858
Target:  5'- uGAGCgUCGGCAucGUCUCCGGCgucGUCa -3'
miRNA:   3'- gUUUG-GGCUGU--CAGAGGCCGaacCAG- -5'
22083 3' -54.6 NC_004927.1 + 53578 0.7 0.592255
Target:  5'- aCAAccuCCCGGCcaaCUCCGGCgagcUGGUCg -3'
miRNA:   3'- -GUUu--GGGCUGucaGAGGCCGa---ACCAG- -5'
22083 3' -54.6 NC_004927.1 + 58708 1.1 0.001488
Target:  5'- uCAAACCCGACAGUCUCCGGCUUGGUCg -3'
miRNA:   3'- -GUUUGGGCUGUCAGAGGCCGAACCAG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.