Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22084 | 5' | -52.6 | NC_004927.1 | + | 60829 | 1.09 | 0.00275 |
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Target: 5'- cUCAACACGCUCAAGCGGUCGAACGCUa -3' miRNA: 3'- -AGUUGUGCGAGUUCGCCAGCUUGCGA- -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 4805 | 0.72 | 0.600878 |
|
Target: 5'- aCAGCACGUUCAgAGCaucuuccagcucucGGUCGAACaGCg -3' miRNA: 3'- aGUUGUGCGAGU-UCG--------------CCAGCUUG-CGa -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 51814 | 0.72 | 0.626089 |
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Target: 5'- gCuuCAgGUUCAGGCGGUCGGugaGCGUg -3' miRNA: 3'- aGuuGUgCGAGUUCGCCAGCU---UGCGa -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 29752 | 0.7 | 0.734402 |
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Target: 5'- cCAGCuuuuCGCugacccauUCGAG-GGUCGAACGCUu -3' miRNA: 3'- aGUUGu---GCG--------AGUUCgCCAGCUUGCGA- -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 55070 | 0.68 | 0.832492 |
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Target: 5'- uUCGAUAC-CUCGGGUGGUUcAGCGUg -3' miRNA: 3'- -AGUUGUGcGAGUUCGCCAGcUUGCGa -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 61430 | 0.68 | 0.841316 |
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Target: 5'- aCGugACGgcgaaUCAGGCaauGGUCGAugGCg -3' miRNA: 3'- aGUugUGCg----AGUUCG---CCAGCUugCGa -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 65600 | 0.67 | 0.881995 |
|
Target: 5'- cUCGACGCugguGUUCcAGCGG-CGAaauACGCUg -3' miRNA: 3'- -AGUUGUG----CGAGuUCGCCaGCU---UGCGA- -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 30923 | 0.67 | 0.881995 |
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Target: 5'- aUCGA-ACGCUCGAuGCGGUCGua-GUa -3' miRNA: 3'- -AGUUgUGCGAGUU-CGCCAGCuugCGa -5' |
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| 22084 | 5' | -52.6 | NC_004927.1 | + | 60377 | 0.66 | 0.889391 |
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Target: 5'- cUCGAUgAUGUUCAAG-GG-CGAGCGCg -3' miRNA: 3'- -AGUUG-UGCGAGUUCgCCaGCUUGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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