Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22109 | 3' | -56.2 | NC_004927.1 | + | 69356 | 0.66 | 0.788001 |
Target: 5'- gACAGCCCGA----AGCCgGuuuCGACCg -3' miRNA: 3'- -UGUUGGGCUgacuUCGGgCu--GCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 72392 | 0.66 | 0.788001 |
Target: 5'- gGCGACCCgGACUcc-GCUCGugGugaagACCCu -3' miRNA: 3'- -UGUUGGG-CUGAcuuCGGGCugC-----UGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 66310 | 0.66 | 0.768762 |
Target: 5'- cGCAcuccGCCgGAUUaaGAAGCCCGGCG-CUa -3' miRNA: 3'- -UGU----UGGgCUGA--CUUCGGGCUGCuGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 33167 | 0.66 | 0.758941 |
Target: 5'- gGCGGgUUGGCgGucGGCCCGGCGuaGCCCu -3' miRNA: 3'- -UGUUgGGCUGaCu-UCGGGCUGC--UGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 46915 | 0.66 | 0.758941 |
Target: 5'- gACAACCCGcgUGAcGCCUuuGACGACg- -3' miRNA: 3'- -UGUUGGGCugACUuCGGG--CUGCUGgg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 21212 | 0.66 | 0.749001 |
Target: 5'- aGCGuCCaCGAUgagGGAcuGCCCGuaggACGACCCg -3' miRNA: 3'- -UGUuGG-GCUGa--CUU--CGGGC----UGCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 67429 | 0.67 | 0.7288 |
Target: 5'- aACGACCCGuGC-GAAGgaCGACGACUa -3' miRNA: 3'- -UGUUGGGC-UGaCUUCggGCUGCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 56087 | 0.67 | 0.724714 |
Target: 5'- cGCGACCCGAUuaUGAAggucgucGUUCGAgggaagcagguucaCGACCCg -3' miRNA: 3'- -UGUUGGGCUG--ACUU-------CGGGCU--------------GCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 69194 | 0.67 | 0.71856 |
Target: 5'- -gAACCCGGCcc--GCCCGAaGACCg -3' miRNA: 3'- ugUUGGGCUGacuuCGGGCUgCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 17496 | 0.67 | 0.70824 |
Target: 5'- aACAGCCCGAacacggcaguCUGAGGgUCGcuACGACUg -3' miRNA: 3'- -UGUUGGGCU----------GACUUCgGGC--UGCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 35788 | 0.68 | 0.676907 |
Target: 5'- aGCcGCCCGACcGAGcuacGUCCGugGaggacGCCCa -3' miRNA: 3'- -UGuUGGGCUGaCUU----CGGGCugC-----UGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 64298 | 0.68 | 0.676907 |
Target: 5'- aGCGGCCCGACUacuaucucGGAGUggacuacgaCGGCGAgCCg -3' miRNA: 3'- -UGUUGGGCUGA--------CUUCGg--------GCUGCUgGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 50813 | 0.68 | 0.655804 |
Target: 5'- aACggUgCGAUUGAAGUCaucaACGGCCCg -3' miRNA: 3'- -UGuuGgGCUGACUUCGGgc--UGCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 45911 | 0.69 | 0.624021 |
Target: 5'- gACGACCUGcucgUGGAGUuuGACGACUa -3' miRNA: 3'- -UGUUGGGCug--ACUUCGggCUGCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 305 | 0.69 | 0.613428 |
Target: 5'- gACGGCCCGcGC-GggGCaggggcaggggCCGAcCGGCCCg -3' miRNA: 3'- -UGUUGGGC-UGaCuuCG-----------GGCU-GCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 75822 | 0.69 | 0.613428 |
Target: 5'- gACGGCCCGcGC-GggGCaggggcaggggCCGAcCGGCCCg -3' miRNA: 3'- -UGUUGGGC-UGaCuuCG-----------GGCU-GCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 15074 | 0.69 | 0.61237 |
Target: 5'- --cGCCCGugUucggagcGAAGCCCGccGCGACUg -3' miRNA: 3'- uguUGGGCugA-------CUUCGGGC--UGCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 21273 | 0.69 | 0.592296 |
Target: 5'- --cAUCCGACUcGucGCCgGGCGACCg -3' miRNA: 3'- uguUGGGCUGA-CuuCGGgCUGCUGGg -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 56461 | 0.69 | 0.570242 |
Target: 5'- aACAGCUCGACagccggaUGAAGCCCGGugggugguuccuUGACUUg -3' miRNA: 3'- -UGUUGGGCUG-------ACUUCGGGCU------------GCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 56153 | 0.7 | 0.56085 |
Target: 5'- gACcACuuGGC-GGcuCCCGACGACCCu -3' miRNA: 3'- -UGuUGggCUGaCUucGGGCUGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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