Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22109 | 3' | -56.2 | NC_004927.1 | + | 8873 | 0.7 | 0.509583 |
Target: 5'- aGCGGCCCGAUU--AGCUCGGCcACCUc -3' miRNA: 3'- -UGUUGGGCUGAcuUCGGGCUGcUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 68618 | 0.72 | 0.450847 |
Target: 5'- cCGACCuCGAUgagaUGGAGgCUGACGACCUu -3' miRNA: 3'- uGUUGG-GCUG----ACUUCgGGCUGCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 67553 | 0.72 | 0.432102 |
Target: 5'- uCAACgaGGCUGAGcGCCCGuuccGCGACCUg -3' miRNA: 3'- uGUUGggCUGACUU-CGGGC----UGCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 61543 | 0.72 | 0.41382 |
Target: 5'- gACu-CCCGGCgcuggcGAGGucuaccucaaCCCGACGACCCg -3' miRNA: 3'- -UGuuGGGCUGa-----CUUC----------GGGCUGCUGGG- -5' |
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22109 | 3' | -56.2 | NC_004927.1 | + | 66477 | 0.8 | 0.1392 |
Target: 5'- gGCGACCCGGCUcaggagGAAGCCCucaauugGGCGACCa -3' miRNA: 3'- -UGUUGGGCUGA------CUUCGGG-------CUGCUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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