Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22164 | 3' | -52.3 | NC_004927.1 | + | 7066 | 0.65 | 0.923524 |
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Target: 5'- cGAAGgacuugagccacaCCGAGAGAcAGCu-UCGCAGAg -3' miRNA: 3'- uCUUCa------------GGCUCUCU-UCGuuGGCGUCU- -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 26565 | 0.66 | 0.919931 |
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Target: 5'- ---cGUCCGGGGu-GGUAGCgGCGGAg -3' miRNA: 3'- ucuuCAGGCUCUcuUCGUUGgCGUCU- -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 20552 | 0.67 | 0.886141 |
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Target: 5'- gAGAAGUgaGAGAGuccAGCAGa-GCAGAa -3' miRNA: 3'- -UCUUCAggCUCUCu--UCGUUggCGUCU- -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 30690 | 0.67 | 0.886141 |
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Target: 5'- cGGGAGUCCGAcucggaaccGAGAGGgAAC-GCGGu -3' miRNA: 3'- -UCUUCAGGCU---------CUCUUCgUUGgCGUCu -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 32771 | 0.67 | 0.87858 |
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Target: 5'- --cGGUgCGAGAGAGGCGgucggcguugacACCGCGc- -3' miRNA: 3'- ucuUCAgGCUCUCUUCGU------------UGGCGUcu -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 23807 | 0.67 | 0.862698 |
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Target: 5'- cAGAGcuGUCCGAGucgucGGAGuCAccACCGUAGAg -3' miRNA: 3'- -UCUU--CAGGCUCu----CUUC-GU--UGGCGUCU- -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 42190 | 0.68 | 0.809507 |
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Target: 5'- uAGGAG-CCGAgcGAGAAGCGcgugGCCGCu-- -3' miRNA: 3'- -UCUUCaGGCU--CUCUUCGU----UGGCGucu -5' |
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| 22164 | 3' | -52.3 | NC_004927.1 | + | 12566 | 0.69 | 0.794095 |
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Target: 5'- uAGAGGUcgccgucccaaagcgCCGAGaAGAAGUGACCGCc-- -3' miRNA: 3'- -UCUUCA---------------GGCUC-UCUUCGUUGGCGucu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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