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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22165 | 3' | -51 | NC_004927.1 | + | 46807 | 0.66 | 0.963073 |
Target: 5'- --aCGCUccgcgagaagugggAggGUCACGAgGGUCUUCGg -3' miRNA: 3'- gaaGCGG--------------UagCAGUGCUaCCAGAAGC- -5' |
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22165 | 3' | -51 | NC_004927.1 | + | 4569 | 0.66 | 0.960466 |
Target: 5'- gCUUCGCUuagGUCGaUACGccGGUCgUCGg -3' miRNA: 3'- -GAAGCGG---UAGCaGUGCuaCCAGaAGC- -5' |
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22165 | 3' | -51 | NC_004927.1 | + | 25412 | 0.66 | 0.952323 |
Target: 5'- gCUUCGUCuUCGUCGCuuucGUGGUCg--- -3' miRNA: 3'- -GAAGCGGuAGCAGUGc---UACCAGaagc -5' |
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22165 | 3' | -51 | NC_004927.1 | + | 56849 | 0.67 | 0.932839 |
Target: 5'- aUUCGUCcUC-UCACGAUGGaCUUCc -3' miRNA: 3'- gAAGCGGuAGcAGUGCUACCaGAAGc -5' |
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22165 | 3' | -51 | NC_004927.1 | + | 11678 | 0.7 | 0.831013 |
Target: 5'- uCUUCGCCGUCcccacUCaucgagccgACGAUGGUgUUCGu -3' miRNA: 3'- -GAAGCGGUAGc----AG---------UGCUACCAgAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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