Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22179 | 5' | -51.4 | NC_004927.1 | + | 35721 | 0.71 | 0.729607 |
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Target: 5'- -cGGUGUGGGCaagaGCGAAG---UCACCc -3' miRNA: 3'- uaCUACACUCGg---CGCUUCaagAGUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 25799 | 0.7 | 0.750807 |
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Target: 5'- aGUGAaccUGAGCCGCGAGG-UCU-ACCu -3' miRNA: 3'- -UACUac-ACUCGGCGCUUCaAGAgUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 43236 | 0.7 | 0.761227 |
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Target: 5'- ---uUGUaGAGCCGCGAAGUaUUCcCCg -3' miRNA: 3'- uacuACA-CUCGGCGCUUCAaGAGuGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 46767 | 0.69 | 0.829731 |
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Target: 5'- uUGAaguugGUGAGCCGaUGGAGcUUCcCGCCg -3' miRNA: 3'- uACUa----CACUCGGC-GCUUC-AAGaGUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 45334 | 0.69 | 0.829731 |
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Target: 5'- -cGGUGUGucCCGagugcuGGUUCUCGCCg -3' miRNA: 3'- uaCUACACucGGCgcu---UCAAGAGUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 2267 | 0.68 | 0.847546 |
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Target: 5'- gAUGAUGUGAaccUCGCGggGcgcUUCgCACCa -3' miRNA: 3'- -UACUACACUc--GGCGCuuC---AAGaGUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 26065 | 0.68 | 0.856111 |
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Target: 5'- -cGAUGUGuucuGCCGCG---UUCUgACCg -3' miRNA: 3'- uaCUACACu---CGGCGCuucAAGAgUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 47479 | 0.67 | 0.915423 |
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Target: 5'- -cGAUacGUGGGCCGauGAGUUUgaaCACCa -3' miRNA: 3'- uaCUA--CACUCGGCgcUUCAAGa--GUGG- -5' |
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| 22179 | 5' | -51.4 | NC_004927.1 | + | 73040 | 0.65 | 0.94689 |
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Target: 5'- cUGGUauUGAGCCaGCGAGGUaucugauagaggacUCUCGCa -3' miRNA: 3'- uACUAc-ACUCGG-CGCUUCA--------------AGAGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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