Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22184 | 5' | -57.1 | NC_004927.1 | + | 43889 | 0.66 | 0.702807 |
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Target: 5'- gAAGGCCcacuAUCCUCAUCcacGUuCCCUuuGa -3' miRNA: 3'- gUUCCGG----UGGGAGUAG---CAuGGGAggC- -5' |
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| 22184 | 5' | -57.1 | NC_004927.1 | + | 21451 | 0.67 | 0.65015 |
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Target: 5'- cCGAGGCuCACCgg-GUCGUcGCCCUCaCGg -3' miRNA: 3'- -GUUCCG-GUGGgagUAGCA-UGGGAG-GC- -5' |
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| 22184 | 5' | -57.1 | NC_004927.1 | + | 3304 | 0.67 | 0.618293 |
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Target: 5'- gUAAGGCCggaGCCUUCGUau--UCCUCCGg -3' miRNA: 3'- -GUUCCGG---UGGGAGUAgcauGGGAGGC- -5' |
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| 22184 | 5' | -57.1 | NC_004927.1 | + | 72394 | 0.68 | 0.586536 |
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Target: 5'- -cGGGCgACCCggacuccgcUCGUgGUgaagACCCUCCGa -3' miRNA: 3'- guUCCGgUGGG---------AGUAgCA----UGGGAGGC- -5' |
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| 22184 | 5' | -57.1 | NC_004927.1 | + | 72316 | 0.74 | 0.295707 |
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Target: 5'- aGAGcGCCACCCUCGaugCGUACCUaCCc -3' miRNA: 3'- gUUC-CGGUGGGAGUa--GCAUGGGaGGc -5' |
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| 22184 | 5' | -57.1 | NC_004927.1 | + | 32178 | 0.74 | 0.288645 |
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Target: 5'- aCGAGGCCGgUCUUGucggugucuUCGUACCCUUCGa -3' miRNA: 3'- -GUUCCGGUgGGAGU---------AGCAUGGGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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