Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22185 | 5' | -58.5 | NC_004927.1 | + | 327 | 0.7 | 0.452497 |
Target: 5'- aCGggCAGGGGGCGGggGGAGaGACg -3' miRNA: 3'- aGCagGUCUCCCGCCagCCUCaCUGg -5' |
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22185 | 5' | -58.5 | NC_004927.1 | + | 32772 | 0.68 | 0.560532 |
Target: 5'- cCGgugCgAGAGaGGCGGUCGGcGuUGACa -3' miRNA: 3'- aGCa--GgUCUC-CCGCCAGCCuC-ACUGg -5' |
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22185 | 5' | -58.5 | NC_004927.1 | + | 37844 | 0.68 | 0.560532 |
Target: 5'- gUCGUCUaugucGGAGGGCuGGUCGGcuuucGACa -3' miRNA: 3'- -AGCAGG-----UCUCCCG-CCAGCCuca--CUGg -5' |
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22185 | 5' | -58.5 | NC_004927.1 | + | 53653 | 0.67 | 0.581062 |
Target: 5'- cCGUUCAGGGcGUGGagGGAGUcACCu -3' miRNA: 3'- aGCAGGUCUCcCGCCagCCUCAcUGG- -5' |
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22185 | 5' | -58.5 | NC_004927.1 | + | 75844 | 0.7 | 0.452497 |
Target: 5'- aCGggCAGGGGGCGGggGGAGaGACg -3' miRNA: 3'- aGCagGUCUCCCGCCagCCUCaCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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